Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23067 | 3' | -59.8 | NC_005178.1 | + | 6695 | 0.66 | 0.396087 |
Target: 5'- aGCG-CCUGGAccggGCGGccucacugcugaGCCAggaggugCCGCCAu -3' miRNA: 3'- gUGCaGGACCU----CGCC------------UGGUa------GGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 15915 | 0.66 | 0.387065 |
Target: 5'- -cCGUcCCUGGAG-GGACCAgUCCaGCUc -3' miRNA: 3'- guGCA-GGACCUCgCCUGGU-AGG-CGGu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 17981 | 0.66 | 0.387065 |
Target: 5'- gGCGaCgUGGAGCGGuCCAcaaacgcCUGCCAg -3' miRNA: 3'- gUGCaGgACCUCGCCuGGUa------GGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 24543 | 0.66 | 0.387065 |
Target: 5'- aGC-UCCUGGAGCGGGuCgGUCgCGaCCu -3' miRNA: 3'- gUGcAGGACCUCGCCU-GgUAG-GC-GGu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 35689 | 0.66 | 0.38617 |
Target: 5'- gUugGUCCUauccgcuggccgaGGAcaugGCGGAUgGUCCGCUc -3' miRNA: 3'- -GugCAGGA-------------CCU----CGCCUGgUAGGCGGu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 17245 | 0.66 | 0.377299 |
Target: 5'- gAUGUCCUGGAGCacauccaGGcgugacgccuugGCCA-CCGUCAg -3' miRNA: 3'- gUGCAGGACCUCG-------CC------------UGGUaGGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 19902 | 0.66 | 0.360826 |
Target: 5'- gAUGUgCCgGGcuGGCGGAUCGaugCCGCCAg -3' miRNA: 3'- gUGCA-GGaCC--UCGCCUGGUa--GGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 16388 | 0.66 | 0.360826 |
Target: 5'- cUACGcCCUGGGGCaGGuCCAUaacgaggugCGCCAu -3' miRNA: 3'- -GUGCaGGACCUCG-CCuGGUAg--------GCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 10305 | 0.66 | 0.360826 |
Target: 5'- gCACGUCCUGGcggauAGCcgaGGGCCGggCGCgGa -3' miRNA: 3'- -GUGCAGGACC-----UCG---CCUGGUagGCGgU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 7480 | 0.66 | 0.355729 |
Target: 5'- cCugG-CCUGGAGCGaACCGaucagcuaccgcgacUUCGCCGg -3' miRNA: 3'- -GugCaGGACCUCGCcUGGU---------------AGGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 21284 | 0.67 | 0.335852 |
Target: 5'- uGCGgccggCCggGGAGCGGguuucgguGCCGUCCGUg- -3' miRNA: 3'- gUGCa----GGa-CCUCGCC--------UGGUAGGCGgu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 31668 | 0.67 | 0.312163 |
Target: 5'- cCAgGUggCCUGGAcgggGCGGACCAggUCCagGCCGg -3' miRNA: 3'- -GUgCA--GGACCU----CGCCUGGU--AGG--CGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 3043 | 0.67 | 0.312163 |
Target: 5'- gGCGcCCUGGuGCcGAagGUCCGCCGc -3' miRNA: 3'- gUGCaGGACCuCGcCUggUAGGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 33612 | 0.67 | 0.303801 |
Target: 5'- -cCGUCCUGGAGaaaaGCCAUCCaauuuagGCCAu -3' miRNA: 3'- guGCAGGACCUCgcc-UGGUAGG-------CGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 15698 | 0.68 | 0.297088 |
Target: 5'- -uCGgCCUGGAcUGGACCAUcgagCCGCCGc -3' miRNA: 3'- guGCaGGACCUcGCCUGGUA----GGCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 6878 | 0.68 | 0.282584 |
Target: 5'- -uCGgCCUGGGGC-GAgCGUCCGCCc -3' miRNA: 3'- guGCaGGACCUCGcCUgGUAGGCGGu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 7777 | 0.68 | 0.275544 |
Target: 5'- --aGUCCUGGuGCGaGGCCAaCCguGCCGa -3' miRNA: 3'- gugCAGGACCuCGC-CUGGUaGG--CGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 30764 | 0.68 | 0.268646 |
Target: 5'- gACGcCUUGGAGCuGAaccaggaCAUCCGCCu -3' miRNA: 3'- gUGCaGGACCUCGcCUg------GUAGGCGGu -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 3116 | 0.68 | 0.261888 |
Target: 5'- uCAUGUCCgGGcGGCGGACCuUCggcaccagggCGCCAc -3' miRNA: 3'- -GUGCAGGaCC-UCGCCUGGuAG----------GCGGU- -5' |
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23067 | 3' | -59.8 | NC_005178.1 | + | 28216 | 0.69 | 0.248787 |
Target: 5'- gCACGcUCCUG-AGCGGcGCCG-CCGCUAu -3' miRNA: 3'- -GUGC-AGGACcUCGCC-UGGUaGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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