Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23067 | 3' | -59.8 | NC_005178.1 | + | 31668 | 0.67 | 0.312163 |
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Target: 5'- cCAgGUggCCUGGAcgggGCGGACCAggUCCagGCCGg -3' miRNA: 3'- -GUgCA--GGACCU----CGCCUGGU--AGG--CGGU- -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 33612 | 0.67 | 0.303801 |
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Target: 5'- -cCGUCCUGGAGaaaaGCCAUCCaauuuagGCCAu -3' miRNA: 3'- guGCAGGACCUCgcc-UGGUAGG-------CGGU- -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 3116 | 0.68 | 0.261888 |
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Target: 5'- uCAUGUCCgGGcGGCGGACCuUCggcaccagggCGCCAc -3' miRNA: 3'- -GUGCAGGaCC-UCGCCUGGuAG----------GCGGU- -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 35008 | 0.69 | 0.236233 |
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Target: 5'- uCACGUCCaGGAGCuggagauuugccGGAUCgAUCaCGCCGg -3' miRNA: 3'- -GUGCAGGaCCUCG------------CCUGG-UAG-GCGGU- -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 20968 | 0.72 | 0.153952 |
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Target: 5'- gGCGUCCUGGAGCGGgguguuGCgGUCgGUg- -3' miRNA: 3'- gUGCAGGACCUCGCC------UGgUAGgCGgu -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 35778 | 1.07 | 0.00029 |
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Target: 5'- gCACGUCCUGGAGCGGACCAUCCGCCAu -3' miRNA: 3'- -GUGCAGGACCUCGCCUGGUAGGCGGU- -5' |
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| 23067 | 3' | -59.8 | NC_005178.1 | + | 15915 | 0.66 | 0.387065 |
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Target: 5'- -cCGUcCCUGGAG-GGACCAgUCCaGCUc -3' miRNA: 3'- guGCA-GGACCUCgCCUGGU-AGG-CGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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