Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23068 | 3' | -56.3 | NC_005178.1 | + | 8491 | 0.66 | 0.596796 |
Target: 5'- cCGugCCGCCCaucagGCGAa-GGUUGu -3' miRNA: 3'- cGCugGGCGGGgaaa-UGCUggCCAAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 2262 | 0.66 | 0.590156 |
Target: 5'- -aGACCCgaGCCCCggccagaugcuUGGCCGGUa- -3' miRNA: 3'- cgCUGGG--CGGGGaaau-------GCUGGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 27222 | 0.66 | 0.57472 |
Target: 5'- cGCGcUUCGCCCCUccGCGcCCGGc-- -3' miRNA: 3'- -CGCuGGGCGGGGAaaUGCuGGCCaac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 20229 | 0.66 | 0.57472 |
Target: 5'- gGCGACCUcgGCCCCgc--CGuCCGGc-- -3' miRNA: 3'- -CGCUGGG--CGGGGaaauGCuGGCCaac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 4925 | 0.66 | 0.56375 |
Target: 5'- aCGGCaCCGCUCg---GCGACCGGg-- -3' miRNA: 3'- cGCUG-GGCGGGgaaaUGCUGGCCaac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 8662 | 0.67 | 0.488951 |
Target: 5'- -aGGCCUGCCCagccGCGACCaGGUcUGg -3' miRNA: 3'- cgCUGGGCGGGgaaaUGCUGG-CCA-AC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 3372 | 0.67 | 0.488951 |
Target: 5'- aGCGGCCCGCCagcucgccucguCCaUUcGCGugCGGg-- -3' miRNA: 3'- -CGCUGGGCGG------------GG-AAaUGCugGCCaac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 26961 | 0.68 | 0.468416 |
Target: 5'- uGCgGugCCGgCUCgcu-CGGCCGGUUGa -3' miRNA: 3'- -CG-CugGGCgGGGaaauGCUGGCCAAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 13115 | 0.68 | 0.458316 |
Target: 5'- cCGGCgCGCCgCCUU--CGGCCaGGUUGa -3' miRNA: 3'- cGCUGgGCGG-GGAAauGCUGG-CCAAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 14187 | 0.69 | 0.41913 |
Target: 5'- cCGACugCCGCCCUggUGCGAcgccCCGGUg- -3' miRNA: 3'- cGCUG--GGCGGGGaaAUGCU----GGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 33701 | 0.71 | 0.31471 |
Target: 5'- cGCGGCaaGCUgCUgUGCGGCCGGUc- -3' miRNA: 3'- -CGCUGggCGGgGAaAUGCUGGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 3023 | 0.72 | 0.277389 |
Target: 5'- aCGAgCgCCGCCCCUgcgacgcaGCGACCGcGUUGu -3' miRNA: 3'- cGCU-G-GGCGGGGAaa------UGCUGGC-CAAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 1584 | 1.11 | 0.000419 |
Target: 5'- uGCGACCCGCCCCUUUACGACCGGUUGg -3' miRNA: 3'- -CGCUGGGCGGGGAAAUGCUGGCCAAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 35110 | 0.66 | 0.56375 |
Target: 5'- cCGAgCUGCCgCUg-GCGGCCGGUc- -3' miRNA: 3'- cGCUgGGCGGgGAaaUGCUGGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 11978 | 0.66 | 0.552837 |
Target: 5'- cCGGCCUaGCCCUggUGCGgGCUGGUa- -3' miRNA: 3'- cGCUGGG-CGGGGaaAUGC-UGGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 33896 | 0.66 | 0.543069 |
Target: 5'- gGCGuCCUGCCgcgCCUggcggcgaugcucgGCGGCCGGUg- -3' miRNA: 3'- -CGCuGGGCGG---GGAaa------------UGCUGGCCAac -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 21604 | 0.67 | 0.509897 |
Target: 5'- --uACUCGCCCCaggcgaagAUGGCCGGUUa -3' miRNA: 3'- cgcUGGGCGGGGaaa-----UGCUGGCCAAc -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 23473 | 0.68 | 0.478629 |
Target: 5'- cGCGaaGCCgGCCCgCUgcuCGGCCGGcUGc -3' miRNA: 3'- -CGC--UGGgCGGG-GAaauGCUGGCCaAC- -5' |
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23068 | 3' | -56.3 | NC_005178.1 | + | 3377 | 0.69 | 0.409654 |
Target: 5'- aCGGCCgGCCCUUc--CGGCCGGa-- -3' miRNA: 3'- cGCUGGgCGGGGAaauGCUGGCCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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