Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23069 | 3' | -54.3 | NC_005178.1 | + | 28261 | 0.66 | 0.677797 |
Target: 5'- cGCUGGCcgccgccaAUGGugucAGUGGGGUGA-CCGGc -3' miRNA: 3'- -CGGCCG--------UACU----UUACCUCGCUaGGCCa -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 10328 | 0.66 | 0.666472 |
Target: 5'- gGCCgGGCGcGGAggGGcgaAGCGcgCCGGg -3' miRNA: 3'- -CGG-CCGUaCUUuaCC---UCGCuaGGCCa -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 32928 | 0.66 | 0.655112 |
Target: 5'- cGCCGGCAUGccGAUGGcuGGUuuaCCGGg -3' miRNA: 3'- -CGGCCGUACu-UUACC--UCGcuaGGCCa -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 26467 | 0.67 | 0.60956 |
Target: 5'- uGCCGGCc-GAGGUGGAuGCGGgaucaggcaUuuGGUg -3' miRNA: 3'- -CGGCCGuaCUUUACCU-CGCU---------AggCCA- -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 13870 | 0.67 | 0.575576 |
Target: 5'- uGCCGGCAUG----GGuGUGAUUgGGUu -3' miRNA: 3'- -CGGCCGUACuuuaCCuCGCUAGgCCA- -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 14641 | 0.68 | 0.553154 |
Target: 5'- aGCgGGCGUGcAGGUGGAGauCGAucUCCGcGUc -3' miRNA: 3'- -CGgCCGUAC-UUUACCUC--GCU--AGGC-CA- -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 10452 | 0.68 | 0.520042 |
Target: 5'- cGCUGGUAc--GGUGGcGCGAUCUGGa -3' miRNA: 3'- -CGGCCGUacuUUACCuCGCUAGGCCa -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 25360 | 0.69 | 0.477178 |
Target: 5'- aGCCaGGC-UGGccgGGAGCuGUCCGGUu -3' miRNA: 3'- -CGG-CCGuACUuuaCCUCGcUAGGCCA- -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 16331 | 0.69 | 0.466733 |
Target: 5'- gGCCGGCcgG----GGAGCGGguUUCGGUg -3' miRNA: 3'- -CGGCCGuaCuuuaCCUCGCU--AGGCCA- -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 33074 | 0.73 | 0.265348 |
Target: 5'- uGCCGGUAgcgcUGGGcgGGAGCGGUCggcaGGg -3' miRNA: 3'- -CGGCCGU----ACUUuaCCUCGCUAGg---CCa -5' |
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23069 | 3' | -54.3 | NC_005178.1 | + | 826 | 1.09 | 0.000734 |
Target: 5'- cGCCGGCAUGAAAUGGAGCGAUCCGGUu -3' miRNA: 3'- -CGGCCGUACUUUACCUCGCUAGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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