Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23073 | 5' | -62.3 | NC_005178.1 | + | 18101 | 0.66 | 0.300386 |
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Target: 5'- gCGCCUGGcCcAG-CCCGCCaugGuGCUGu -3' miRNA: 3'- -GCGGACCaGcUCaGGGCGGg--CuCGAC- -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 32240 | 0.66 | 0.293138 |
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Target: 5'- cCGCCUGGUCGAG--CCGaCCGAuGUa- -3' miRNA: 3'- -GCGGACCAGCUCagGGCgGGCU-CGac -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 10797 | 0.67 | 0.258944 |
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Target: 5'- -uCCUGGcCGAG-CCCaGCCgCGAGCg- -3' miRNA: 3'- gcGGACCaGCUCaGGG-CGG-GCUCGac -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 12595 | 0.68 | 0.210545 |
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Target: 5'- cCGCCUGGagcgcuUCGAGgaccgauUCCCGCagauCGAGCg- -3' miRNA: 3'- -GCGGACC------AGCUC-------AGGGCGg---GCUCGac -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 14878 | 0.68 | 0.200382 |
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Target: 5'- gGCCUGGgCGAGg-UCGCCCuGGGCUu -3' miRNA: 3'- gCGGACCaGCUCagGGCGGG-CUCGAc -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 23225 | 0.69 | 0.180376 |
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Target: 5'- -uCCUGGUCGAGcgcaUCCGCgCCGAGgUGc -3' miRNA: 3'- gcGGACCAGCUCa---GGGCG-GGCUCgAC- -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 7777 | 0.7 | 0.149604 |
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Target: 5'- aGuCCUGGUgCGAGgccaaCCGUgCCGAGCUGa -3' miRNA: 3'- gC-GGACCA-GCUCag---GGCG-GGCUCGAC- -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 6880 | 0.73 | 0.086283 |
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Target: 5'- gGCCUGGggCGAGcgUCCGCCCGucuucguccAGCUGc -3' miRNA: 3'- gCGGACCa-GCUCa-GGGCGGGC---------UCGAC- -5' |
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| 23073 | 5' | -62.3 | NC_005178.1 | + | 1592 | 1.08 | 0.000148 |
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Target: 5'- aCGCCUGGUCGAGUCCCGCCCGAGCUGa -3' miRNA: 3'- -GCGGACCAGCUCAGGGCGGGCUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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