Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23075 | 5' | -64.2 | NC_005178.1 | + | 25315 | 0.66 | 0.234064 |
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Target: 5'- uUGGUCGCUGucgGCCCgcuugGCCAGGcgcuGGa -3' miRNA: 3'- gACCAGCGGCua-CGGG-----CGGUCCc---CCa -5' |
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| 23075 | 5' | -64.2 | NC_005178.1 | + | 16430 | 0.66 | 0.200657 |
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Target: 5'- aCUGGcUGCgGAUGCCCGCCAGc---- -3' miRNA: 3'- -GACCaGCGgCUACGGGCGGUCcccca -5' |
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| 23075 | 5' | -64.2 | NC_005178.1 | + | 36529 | 0.69 | 0.142268 |
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Target: 5'- aUGGgacgGCCGAgcgguagccuUGCCCGCUAcGGGGUg -3' miRNA: 3'- gACCag--CGGCU----------ACGGGCGGUcCCCCA- -5' |
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| 23075 | 5' | -64.2 | NC_005178.1 | + | 11374 | 0.69 | 0.131201 |
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Target: 5'- cCUGGUgauUGCCGGucUGCCCGCCuGGuGGc -3' miRNA: 3'- -GACCA---GCGGCU--ACGGGCGGuCCcCCa -5' |
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| 23075 | 5' | -64.2 | NC_005178.1 | + | 6797 | 0.71 | 0.098448 |
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Target: 5'- cCUGGUCGgUGAUGCCCugcugccgauccgccGCCucauugccuggcaguGGGGGUg -3' miRNA: 3'- -GACCAGCgGCUACGGG---------------CGGu--------------CCCCCA- -5' |
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| 23075 | 5' | -64.2 | NC_005178.1 | + | 2809 | 1.07 | 0.000113 |
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Target: 5'- aCUGGUCGCCGAUGCCCGCCAGGGGGUg -3' miRNA: 3'- -GACCAGCGGCUACGGGCGGUCCCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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