miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23075 5' -64.2 NC_005178.1 + 25315 0.66 0.234064
Target:  5'- uUGGUCGCUGucgGCCCgcuugGCCAGGcgcuGGa -3'
miRNA:   3'- gACCAGCGGCua-CGGG-----CGGUCCc---CCa -5'
23075 5' -64.2 NC_005178.1 + 16430 0.66 0.200657
Target:  5'- aCUGGcUGCgGAUGCCCGCCAGc---- -3'
miRNA:   3'- -GACCaGCGgCUACGGGCGGUCcccca -5'
23075 5' -64.2 NC_005178.1 + 36529 0.69 0.142268
Target:  5'- aUGGgacgGCCGAgcgguagccuUGCCCGCUAcGGGGUg -3'
miRNA:   3'- gACCag--CGGCU----------ACGGGCGGUcCCCCA- -5'
23075 5' -64.2 NC_005178.1 + 11374 0.69 0.131201
Target:  5'- cCUGGUgauUGCCGGucUGCCCGCCuGGuGGc -3'
miRNA:   3'- -GACCA---GCGGCU--ACGGGCGGuCCcCCa -5'
23075 5' -64.2 NC_005178.1 + 6797 0.71 0.098448
Target:  5'- cCUGGUCGgUGAUGCCCugcugccgauccgccGCCucauugccuggcaguGGGGGUg -3'
miRNA:   3'- -GACCAGCgGCUACGGG---------------CGGu--------------CCCCCA- -5'
23075 5' -64.2 NC_005178.1 + 2809 1.07 0.000113
Target:  5'- aCUGGUCGCCGAUGCCCGCCAGGGGGUg -3'
miRNA:   3'- -GACCAGCGGCUACGGGCGGUCCCCCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.