Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23076 | 5' | -54.6 | NC_005178.1 | + | 7740 | 0.66 | 0.597354 |
Target: 5'- cUGaAGAAGCGcCUGGCGGuccuccaGGGCGGAg- -3' miRNA: 3'- -ACcUCUUCGU-GACCGUC-------CCCGUUUac -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 6689 | 0.66 | 0.575506 |
Target: 5'- aGGGGGAGCGcCUGGaccGGGCGGc-- -3' miRNA: 3'- aCCUCUUCGU-GACCgucCCCGUUuac -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 12660 | 0.67 | 0.55271 |
Target: 5'- cGGccGgcGCGCUGGCGGGuGGCc---- -3' miRNA: 3'- aCCu-CuuCGUGACCGUCC-CCGuuuac -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 12092 | 0.67 | 0.541413 |
Target: 5'- gUGGAGuGGuCGCgUGGCGGGccuGGCcGAUGg -3' miRNA: 3'- -ACCUCuUC-GUG-ACCGUCC---CCGuUUAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 9076 | 0.67 | 0.530193 |
Target: 5'- cGGAGuAAGCcCUGGCuGcauGGCGGAUGc -3' miRNA: 3'- aCCUC-UUCGuGACCGuCc--CCGUUUAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 15335 | 0.67 | 0.530193 |
Target: 5'- aUGGAGuucGCcCUGGUAGGccGGCAAGg- -3' miRNA: 3'- -ACCUCuu-CGuGACCGUCC--CCGUUUac -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 22195 | 0.67 | 0.508019 |
Target: 5'- aUGGcuccaccGGCACUGGCgcauaaGGGGGCAGcucAUGg -3' miRNA: 3'- -ACCucu----UCGUGACCG------UCCCCGUU---UAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 36104 | 0.68 | 0.444107 |
Target: 5'- gGGAGuacaugccgcccAGGUuggGCUGGCGGGuGCAGGUGg -3' miRNA: 3'- aCCUC------------UUCG---UGACCGUCCcCGUUUAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 17281 | 0.69 | 0.433894 |
Target: 5'- cUGGAGGccaaAG-GCUGGUGGGGGCGucagauAGUGg -3' miRNA: 3'- -ACCUCU----UCgUGACCGUCCCCGU------UUAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 8162 | 0.7 | 0.357411 |
Target: 5'- cGGAGAuagcagcuGCGCUcGUAGGGGCugcuGAUGc -3' miRNA: 3'- aCCUCUu-------CGUGAcCGUCCCCGu---UUAC- -5' |
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23076 | 5' | -54.6 | NC_005178.1 | + | 3160 | 1.08 | 0.000694 |
Target: 5'- cUGGAGAAGCACUGGCAGGGGCAAAUGc -3' miRNA: 3'- -ACCUCUUCGUGACCGUCCCCGUUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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