Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23077 | 3' | -62.2 | NC_005178.1 | + | 8670 | 0.66 | 0.27241 |
Target: 5'- cCAGCCGCGAccaGGUCuGGGUucGCgGUGaGGa -3' miRNA: 3'- aGUCGGCGCU---CCAG-CCCG--CG-UAC-CCa -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 8804 | 0.66 | 0.275149 |
Target: 5'- -gAGCCGCaccAGGUCGgcaagaaagucgcccGGCGCGUgccGGGUg -3' miRNA: 3'- agUCGGCGc--UCCAGC---------------CCGCGUA---CCCA- -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 18339 | 0.66 | 0.279299 |
Target: 5'- -uGGaCCGCGAGGUCcaGGaGCGCcUGGcGg -3' miRNA: 3'- agUC-GGCGCUCCAG--CC-CGCGuACC-Ca -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 21239 | 0.66 | 0.286326 |
Target: 5'- cCGGCCGCaucaucGGcGUCGaaccggacGGCGUGUGGGUg -3' miRNA: 3'- aGUCGGCGc-----UC-CAGC--------CCGCGUACCCA- -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 34596 | 0.66 | 0.27241 |
Target: 5'- gCGGCCGCGAGGaucgCGGGC-CAUc--- -3' miRNA: 3'- aGUCGGCGCUCCa---GCCCGcGUAccca -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 8855 | 0.68 | 0.216447 |
Target: 5'- gUCGGCCGCcaucuGGUCGGGgucuugaacaGCAUGGa- -3' miRNA: 3'- -AGUCGGCGcu---CCAGCCCg---------CGUACCca -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 24756 | 0.68 | 0.218143 |
Target: 5'- gCGGCCGCGAccuccagggacugccGGUCGaggacguaggcgcGGCGCA-GGGc -3' miRNA: 3'- aGUCGGCGCU---------------CCAGC-------------CCGCGUaCCCa -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 10319 | 0.7 | 0.137382 |
Target: 5'- aUAGCCGa-GGGcCGGGCGCGgagGGGc -3' miRNA: 3'- aGUCGGCgcUCCaGCCCGCGUa--CCCa -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 18877 | 0.71 | 0.123087 |
Target: 5'- -aGGaCCGgGAGGUaCGGGCGCGccGGGa -3' miRNA: 3'- agUC-GGCgCUCCA-GCCCGCGUa-CCCa -5' |
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23077 | 3' | -62.2 | NC_005178.1 | + | 3232 | 1.07 | 0.000163 |
Target: 5'- gUCAGCCGCGAGGUCGGGCGCAUGGGUg -3' miRNA: 3'- -AGUCGGCGCUCCAGCCCGCGUACCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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