Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23077 | 5' | -54.5 | NC_005178.1 | + | 16547 | 0.69 | 0.45454 |
Target: 5'- --cGGUGCUGcCGCCCacggcgaacccgGUagCGUUCGCCa -3' miRNA: 3'- guuCCGUGAC-GCGGG------------CAa-GUAAGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 20150 | 0.69 | 0.434299 |
Target: 5'- --cGGCAUaGCGCCCGgcCAUguagGCCg -3' miRNA: 3'- guuCCGUGaCGCGGGCaaGUAag--CGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 4002 | 0.7 | 0.424376 |
Target: 5'- -cAGGUGCUGCGCaCCGUc----CGCCg -3' miRNA: 3'- guUCCGUGACGCG-GGCAaguaaGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 6302 | 0.7 | 0.420446 |
Target: 5'- uCGAGGCGCUcagGCGCUCGacgaugcugUCGCCc -3' miRNA: 3'- -GUUCCGUGA---CGCGGGCaagua----AGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 29156 | 0.71 | 0.376872 |
Target: 5'- -cAGGCGCUGCuguggaaucaGCCCaugccguacugGUUC-UUCGCCa -3' miRNA: 3'- guUCCGUGACG----------CGGG-----------CAAGuAAGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 25696 | 0.71 | 0.350142 |
Target: 5'- --cGGCGCUGUagggcugcuGCCCGUUUucgUGCCg -3' miRNA: 3'- guuCCGUGACG---------CGGGCAAGuaaGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 27993 | 0.72 | 0.300824 |
Target: 5'- gCAGGGCAUcGCGCUCGcgC-UUCGCUc -3' miRNA: 3'- -GUUCCGUGaCGCGGGCaaGuAAGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 23662 | 0.73 | 0.263962 |
Target: 5'- ---cGCGCUG-GCCCGUUCA--CGCCg -3' miRNA: 3'- guucCGUGACgCGGGCAAGUaaGCGG- -5' |
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23077 | 5' | -54.5 | NC_005178.1 | + | 3269 | 1.12 | 0.000416 |
Target: 5'- uCAAGGCACUGCGCCCGUUCAUUCGCCg -3' miRNA: 3'- -GUUCCGUGACGCGGGCAAGUAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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