Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23079 | 5' | -55.2 | NC_005178.1 | + | 17801 | 0.66 | 0.591867 |
Target: 5'- aUGCCGC-CAUgGUggcGACggUCGAGCGCg -3' miRNA: 3'- aACGGCGcGUAgCAa--CUG--GGCUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 352 | 0.66 | 0.591867 |
Target: 5'- cUGCCGCGUcuuggacCGUUGACuuGGugugauccacaaGCACu -3' miRNA: 3'- aACGGCGCGua-----GCAACUGggCU------------UGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 18152 | 0.66 | 0.588448 |
Target: 5'- -gGCUGCGCGcCGUUGGCUgaugccggcuuucuUGAGCAg -3' miRNA: 3'- aaCGGCGCGUaGCAACUGG--------------GCUUGUg -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 33791 | 0.66 | 0.580484 |
Target: 5'- gUGCCGCcCuuggCGaUGGCCCG-GCGCg -3' miRNA: 3'- aACGGCGcGua--GCaACUGGGCuUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 11437 | 0.67 | 0.524444 |
Target: 5'- -aGCCGUcuugGCGUUGUUGGCC--GACGCu -3' miRNA: 3'- aaCGGCG----CGUAGCAACUGGgcUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 11743 | 0.67 | 0.491812 |
Target: 5'- --cCCGCGCAUaugccaCGgccgUGGCCCGAGC-Ca -3' miRNA: 3'- aacGGCGCGUA------GCa---ACUGGGCUUGuG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 22635 | 0.67 | 0.481142 |
Target: 5'- -cGCCGCGCAga----ACgCCGAGCACa -3' miRNA: 3'- aaCGGCGCGUagcaacUG-GGCUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 23250 | 0.68 | 0.429601 |
Target: 5'- -gGCCGCGCAUCcgcuugccgGAgCCGAAgACc -3' miRNA: 3'- aaCGGCGCGUAGcaa------CUgGGCUUgUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 34193 | 0.69 | 0.419686 |
Target: 5'- -gGCCGUGCAUCGg-GugCUGGACc- -3' miRNA: 3'- aaCGGCGCGUAGCaaCugGGCUUGug -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 9934 | 0.69 | 0.419686 |
Target: 5'- -gGCCaGCGCAUCcuGUUGuuGCUgGAGCGCg -3' miRNA: 3'- aaCGG-CGCGUAG--CAAC--UGGgCUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 34001 | 0.69 | 0.372265 |
Target: 5'- -cGUCGCGCAUCaugugGUUGacguaGCCCGAGC-Cg -3' miRNA: 3'- aaCGGCGCGUAG-----CAAC-----UGGGCUUGuG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 34694 | 0.7 | 0.363228 |
Target: 5'- -aGCCGUuccGCAUcCGUUcGACCCG-GCGCg -3' miRNA: 3'- aaCGGCG---CGUA-GCAA-CUGGGCuUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 1216 | 0.7 | 0.331133 |
Target: 5'- -cGCCGCGCuggaccugagcccUGUgaagcucUGGCCCGAACGCu -3' miRNA: 3'- aaCGGCGCGua-----------GCA-------ACUGGGCUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 20427 | 0.72 | 0.274075 |
Target: 5'- -aGCUGCGCGaaacccUCGgccUGGCCCaGGACGCg -3' miRNA: 3'- aaCGGCGCGU------AGCa--ACUGGG-CUUGUG- -5' |
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23079 | 5' | -55.2 | NC_005178.1 | + | 3867 | 1.06 | 0.000857 |
Target: 5'- cUUGCCGCGCAUCGUUGACCCGAACACc -3' miRNA: 3'- -AACGGCGCGUAGCAACUGGGCUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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