Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23080 | 3' | -59.1 | NC_005178.1 | + | 23428 | 0.66 | 0.437025 |
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Target: 5'- aCGUUGUacuacgCCGACgCCCAGG-GCgacgGCc -3' miRNA: 3'- gGCGACAa-----GGCUG-GGGUCCaCGa---CGc -5' |
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| 23080 | 3' | -59.1 | NC_005178.1 | + | 18095 | 0.66 | 0.427448 |
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Target: 5'- gCCGCaGcgCCuGGCCCagcccgccauGGUGCUGUGg -3' miRNA: 3'- -GGCGaCaaGG-CUGGGgu--------CCACGACGC- -5' |
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| 23080 | 3' | -59.1 | NC_005178.1 | + | 22943 | 0.68 | 0.330878 |
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Target: 5'- gCCGCUGgcCCaguGCCUguGGUGCUGa- -3' miRNA: 3'- -GGCGACaaGGc--UGGGguCCACGACgc -5' |
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| 23080 | 3' | -59.1 | NC_005178.1 | + | 32393 | 0.68 | 0.330878 |
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Target: 5'- -gGCUGUagaCCGGCgCCAGGUuGCggGCGu -3' miRNA: 3'- ggCGACAa--GGCUGgGGUCCA-CGa-CGC- -5' |
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| 23080 | 3' | -59.1 | NC_005178.1 | + | 20570 | 0.81 | 0.038547 |
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Target: 5'- gCCGUUGagCCGGCCCCAGGUGUcgGCc -3' miRNA: 3'- -GGCGACaaGGCUGGGGUCCACGa-CGc -5' |
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| 23080 | 3' | -59.1 | NC_005178.1 | + | 3987 | 0.97 | 0.002315 |
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Target: 5'- gCCGCU-UUCCGACCCCAGGUGCUGCGc -3' miRNA: 3'- -GGCGAcAAGGCUGGGGUCCACGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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