Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23084 | 5' | -55.3 | NC_005178.1 | + | 25357 | 0.66 | 0.613469 |
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Target: 5'- gCCAGCcaGgCUG-GCCgGGAGCUGUcCGg -3' miRNA: 3'- -GGUCG--CgGACuUGGaCCUCGACAuGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 27738 | 0.84 | 0.040941 |
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Target: 5'- gCAGCGCCUGGACCUGGcgAGCcaGUACGg -3' miRNA: 3'- gGUCGCGGACUUGGACC--UCGa-CAUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 30526 | 0.69 | 0.382036 |
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Target: 5'- -aGGUGCC-GGACUUcGGGCUGUGCAa -3' miRNA: 3'- ggUCGCGGaCUUGGAcCUCGACAUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 31747 | 0.68 | 0.440815 |
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Target: 5'- cCCAGCGCCUGGcccgagguaauGCCggcgacgaccuacagGGGGCggccaGUGCu -3' miRNA: 3'- -GGUCGCGGACU-----------UGGa--------------CCUCGa----CAUGu -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 36193 | 0.69 | 0.39131 |
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Target: 5'- gCCAGUGgCUGGAC--GGAGCUGguagGCGg -3' miRNA: 3'- -GGUCGCgGACUUGgaCCUCGACa---UGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 37223 | 0.7 | 0.345574 |
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Target: 5'- aCAGCGCCgGAccugacaGCCUGGgucgaAGCUGU-CAa -3' miRNA: 3'- gGUCGCGGaCU-------UGGACC-----UCGACAuGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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