Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23084 | 5' | -55.3 | NC_005178.1 | + | 7891 | 0.69 | 0.419995 |
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Target: 5'- gCGGCGCCgaugcGGuCCUGGAGCUacUGCGc -3' miRNA: 3'- gGUCGCGGa----CUuGGACCUCGAc-AUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 7314 | 0.69 | 0.39506 |
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Target: 5'- uCCAGCGCaUGGggguccgccuucaucGCCUGGAGCcg-ACAg -3' miRNA: 3'- -GGUCGCGgACU---------------UGGACCUCGacaUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 6694 | 0.66 | 0.579499 |
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Target: 5'- -gAGCGCCUGGACCgGGcggccucacuGCUGaGCc -3' miRNA: 3'- ggUCGCGGACUUGGaCCu---------CGACaUGu -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 5324 | 1.1 | 0.000486 |
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Target: 5'- uCCAGCGCCUGAACCUGGAGCUGUACAc -3' miRNA: 3'- -GGUCGCGGACUUGGACCUCGACAUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 2186 | 0.7 | 0.363933 |
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Target: 5'- gUCAGCaGCC-GGGCCUGGAGCgucACGc -3' miRNA: 3'- -GGUCG-CGGaCUUGGACCUCGacaUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 2150 | 0.66 | 0.613469 |
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Target: 5'- gCCAGCGCCgGGGCCaguUGGcccucGCUGg--- -3' miRNA: 3'- -GGUCGCGGaCUUGG---ACCu----CGACaugu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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