Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23084 | 5' | -55.3 | NC_005178.1 | + | 17802 | 0.76 | 0.146206 |
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Target: 5'- -gAGCGCCcugaUGGACCaGGuGCUGUACAa -3' miRNA: 3'- ggUCGCGG----ACUUGGaCCuCGACAUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 9752 | 0.77 | 0.130355 |
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Target: 5'- uCCAGCGCCuUGAGCUUGucGUUGUACu -3' miRNA: 3'- -GGUCGCGG-ACUUGGACcuCGACAUGu -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 16989 | 0.78 | 0.109536 |
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Target: 5'- gUCAGCGCCgcgaccUGGcCCUGGAGCUGggugACAg -3' miRNA: 3'- -GGUCGCGG------ACUuGGACCUCGACa---UGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 8410 | 0.83 | 0.047623 |
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Target: 5'- gCAGCGCCUGAACCcGcGGGCUGUAg- -3' miRNA: 3'- gGUCGCGGACUUGGaC-CUCGACAUgu -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 27738 | 0.84 | 0.040941 |
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Target: 5'- gCAGCGCCUGGACCUGGcgAGCcaGUACGg -3' miRNA: 3'- gGUCGCGGACUUGGACC--UCGa-CAUGU- -5' |
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| 23084 | 5' | -55.3 | NC_005178.1 | + | 5324 | 1.1 | 0.000486 |
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Target: 5'- uCCAGCGCCUGAACCUGGAGCUGUACAc -3' miRNA: 3'- -GGUCGCGGACUUGGACCUCGACAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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