Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23085 | 5' | -51 | NC_005178.1 | + | 30506 | 0.66 | 0.864087 |
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Target: 5'- gCGCUcGCGGuAUGUGGGcGAGgug-CCg -3' miRNA: 3'- gGCGGuCGUC-UACACCC-UUCauuaGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 26465 | 0.66 | 0.855405 |
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Target: 5'- gCUGCCGGCcgagguGGAUGcGGGAucaggcauuuGGUGcuUCCg -3' miRNA: 3'- -GGCGGUCG------UCUACaCCCU----------UCAUu-AGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 2747 | 0.66 | 0.846464 |
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Target: 5'- gUCGCCGGCAGAUu---GAAcUGAUCCu -3' miRNA: 3'- -GGCGGUCGUCUAcaccCUUcAUUAGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 15017 | 0.66 | 0.831643 |
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Target: 5'- aUCGCCaugcAGCGGGccuaucacugccuggUGUGGGgcGUugaggcGGUCCa -3' miRNA: 3'- -GGCGG----UCGUCU---------------ACACCCuuCA------UUAGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 1780 | 0.67 | 0.822074 |
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Target: 5'- -aGCCGGCAGGcacgUGaacucaagcgacuuaUGGGAGGU-AUCUg -3' miRNA: 3'- ggCGGUCGUCU----AC---------------ACCCUUCAuUAGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 18114 | 0.68 | 0.741892 |
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Target: 5'- cCCGCCAuGguGcUGUGGGAGcGUGgcagcgagucgcugGUCUa -3' miRNA: 3'- -GGCGGU-CguCuACACCCUU-CAU--------------UAGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 4117 | 0.74 | 0.421976 |
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Target: 5'- aCGCCAGCAgGGUGUGGGucuacgacggcGAGgg--CCg -3' miRNA: 3'- gGCGGUCGU-CUACACCC-----------UUCauuaGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 2447 | 0.76 | 0.313068 |
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Target: 5'- gCCGCCAGCGGcagcucgGcGGcGAGUAGUCCg -3' miRNA: 3'- -GGCGGUCGUCua-----CaCCcUUCAUUAGG- -5' |
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| 23085 | 5' | -51 | NC_005178.1 | + | 5434 | 1.16 | 0.000625 |
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Target: 5'- cCCGCCAGCAGAUGUGGGAAGUAAUCCg -3' miRNA: 3'- -GGCGGUCGUCUACACCCUUCAUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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