Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23087 | 3' | -55.2 | NC_005178.1 | + | 14320 | 0.7 | 0.428737 |
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Target: 5'- gCGGcCUucgacACGGCCCGcaccguCCUGACCuGGu -3' miRNA: 3'- -GCCaGA-----UGCUGGGCuu----GGACUGGuCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 30800 | 0.71 | 0.373122 |
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Target: 5'- gCGGUC-GCGGCCUcGACCUgGGCCGGc -3' miRNA: 3'- -GCCAGaUGCUGGGcUUGGA-CUGGUCc -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 28568 | 0.72 | 0.322615 |
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Target: 5'- -cGUCUACGGCgCCGuAGCuCUGuCCGGGg -3' miRNA: 3'- gcCAGAUGCUG-GGC-UUG-GACuGGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 13477 | 0.73 | 0.284561 |
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Target: 5'- aGGUUgucCGGgCCGAugCUGugCAGGg -3' miRNA: 3'- gCCAGau-GCUgGGCUugGACugGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 25316 | 0.74 | 0.250142 |
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Target: 5'- uGGUCgcuguCGGCCC--GCUUGGCCAGGc -3' miRNA: 3'- gCCAGau---GCUGGGcuUGGACUGGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 5914 | 1.09 | 0.000815 |
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Target: 5'- aGGUCUACGACCCGAACCUGACCAGGu -3' miRNA: 3'- gCCAGAUGCUGGGCUUGGACUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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