Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23087 | 3' | -55.2 | NC_005178.1 | + | 31574 | 0.66 | 0.634564 |
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Target: 5'- aGGUCaAggcagugaccgccgcCGGCCUGGACCUGGuccgccccguCCAGGc -3' miRNA: 3'- gCCAGaU---------------GCUGGGCUUGGACU----------GGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 13860 | 0.66 | 0.652353 |
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Target: 5'- aGGUUU-CGACCCcGACCcgaaGGCCAGc -3' miRNA: 3'- gCCAGAuGCUGGGcUUGGa---CUGGUCc -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 35589 | 0.66 | 0.656794 |
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Target: 5'- aCGGUCggcaacauCGGCCCGGAagCUGGggcgacggugacgcuCCAGGu -3' miRNA: 3'- -GCCAGau------GCUGGGCUUg-GACU---------------GGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 34015 | 0.66 | 0.667879 |
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Target: 5'- uGGUUgACGuaGCCCGAGCCguUGuccacguagaagaugGCCGGGa -3' miRNA: 3'- gCCAGaUGC--UGGGCUUGG--AC---------------UGGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 16310 | 0.66 | 0.67341 |
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Target: 5'- cCGGUU--CGACgCCGAugaugcgGCC-GGCCGGGg -3' miRNA: 3'- -GCCAGauGCUG-GGCU-------UGGaCUGGUCC- -5' |
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| 23087 | 3' | -55.2 | NC_005178.1 | + | 20999 | 0.66 | 0.674515 |
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Target: 5'- gCGGcCagguCGGCCCGGuCCUugaggucgaaGACCAGGc -3' miRNA: 3'- -GCCaGau--GCUGGGCUuGGA----------CUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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