Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 231 | 5' | -57 | AC_000008.1 | + | 5345 | 0.66 | 0.484913 |
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Target: 5'- gUGCgcuccgGGCUGCGCGCuGGCCa- -3' miRNA: 3'- -ACGaacuaaCCGGCGUGCGcCUGGag -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 10010 | 0.66 | 0.443694 |
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Target: 5'- gGCggcgGAguUUGGCCGUAgGUGGcgcCCUCu -3' miRNA: 3'- aCGaa--CU--AACCGGCGUgCGCCu--GGAG- -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 21965 | 0.68 | 0.367602 |
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Target: 5'- gUGCcuUUGAcgacgggUGGgCGgGCGCGGGCCUg -3' miRNA: 3'- -ACG--AACUa------ACCgGCgUGCGCCUGGAg -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 25428 | 0.68 | 0.341472 |
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Target: 5'- cGCUccg-UGGCCGCGCaccugGCGGACaUCa -3' miRNA: 3'- aCGAacuaACCGGCGUG-----CGCCUGgAG- -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 27579 | 0.69 | 0.316711 |
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Target: 5'- aGCUccGAcc-GCCGCGCGCGGACaUCu -3' miRNA: 3'- aCGAa-CUaacCGGCGUGCGCCUGgAG- -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 17245 | 0.7 | 0.285839 |
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Target: 5'- gGCUacg--GGCCGCAacgauCGCGGACCg- -3' miRNA: 3'- aCGAacuaaCCGGCGU-----GCGCCUGGag -5' |
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| 231 | 5' | -57 | AC_000008.1 | + | 18760 | 1.1 | 0.00028 |
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Target: 5'- cUGCUUGAUUGGCCGCACGCGGACCUCg -3' miRNA: 3'- -ACGAACUAACCGGCGUGCGCCUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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