Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23101 | 3' | -58.4 | NC_005178.1 | + | 10354 | 0.66 | 0.409711 |
Target: 5'- -cCGGGCuGCGCUgGCcgagcugGugGGcAGCGa -3' miRNA: 3'- aaGCCCGuCGCGAgCG-------UugCCuUCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 11047 | 1.06 | 0.000402 |
Target: 5'- aUUCGGGCAGCGCUCGCAACGGAAGCAc -3' miRNA: 3'- -AAGCCCGUCGCGAGCGUUGCCUUCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 16373 | 0.67 | 0.338769 |
Target: 5'- gUCGGGCGGCGg-CGCcuACGcccuGggGCAg -3' miRNA: 3'- aAGCCCGUCGCgaGCGu-UGC----CuuCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 23680 | 0.68 | 0.28372 |
Target: 5'- -cCGGGCGGCGCUgGCcuuCGGGucggGGUc -3' miRNA: 3'- aaGCCCGUCGCGAgCGuu-GCCU----UCGu -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 26094 | 0.66 | 0.39182 |
Target: 5'- --gGGGCGGaCGCUgcCGCAGCGucGGCc -3' miRNA: 3'- aagCCCGUC-GCGA--GCGUUGCcuUCGu -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 28526 | 0.69 | 0.248899 |
Target: 5'- -aUGGGCGGCGCUacaUGacugGGCGGggGCc -3' miRNA: 3'- aaGCCCGUCGCGA---GCg---UUGCCuuCGu -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 29903 | 0.66 | 0.373546 |
Target: 5'- -aCGGGCca-GCUCGCGcUGGAGGUc -3' miRNA: 3'- aaGCCCGucgCGAGCGUuGCCUUCGu -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 30405 | 0.69 | 0.241729 |
Target: 5'- uUUCcaGCAGCGCaUCGCGACGGGguuguucAGCGg -3' miRNA: 3'- -AAGccCGUCGCG-AGCGUUGCCU-------UCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 30491 | 0.69 | 0.262386 |
Target: 5'- gUUCGGcgcucaGCAGCGCUCGCGguAUGuGGGCGa -3' miRNA: 3'- -AAGCC------CGUCGCGAGCGU--UGCcUUCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 31311 | 0.67 | 0.347238 |
Target: 5'- -cCGGGCuaugcgccuGGCGC-CGCGcCGGGAGUu -3' miRNA: 3'- aaGCCCG---------UCGCGaGCGUuGCCUUCGu -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 34539 | 0.67 | 0.313474 |
Target: 5'- --gGGGCGGCGCUCGUcugaGGAaaucgaguuccugGGCGu -3' miRNA: 3'- aagCCCGUCGCGAGCGuug-CCU-------------UCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 34837 | 0.73 | 0.131771 |
Target: 5'- --aGGGCGGCGUUCaGCAGCGcGgcGCGg -3' miRNA: 3'- aagCCCGUCGCGAG-CGUUGC-CuuCGU- -5' |
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23101 | 3' | -58.4 | NC_005178.1 | + | 36027 | 0.72 | 0.165248 |
Target: 5'- -gCGGGCGGCGacCUCGUAACGGucaaggucGCAa -3' miRNA: 3'- aaGCCCGUCGC--GAGCGUUGCCuu------CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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