Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23103 | 5' | -61.6 | NC_005178.1 | + | 29301 | 0.66 | 0.312369 |
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Target: 5'- aCCCGcucGCCGUGGuGGGcUCCuaCCGUGGc -3' miRNA: 3'- -GGGCu--CGGUACC-CCCaAGGc-GGCGUC- -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 36724 | 0.66 | 0.300467 |
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Target: 5'- aCCGGaucgcuccauuucauGCCGgcGGGGGUUCCGaCGaCAGa -3' miRNA: 3'- gGGCU---------------CGGUa-CCCCCAAGGCgGC-GUC- -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 17118 | 0.68 | 0.231594 |
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Target: 5'- uCCUGGGCCA-GGccgaGGGUUUCG-CGCAGc -3' miRNA: 3'- -GGGCUCGGUaCC----CCCAAGGCgGCGUC- -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 7304 | 0.69 | 0.203493 |
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Target: 5'- gCCCGgcugcuccAGCgCAUGGGGG-UCCGCCu--- -3' miRNA: 3'- -GGGC--------UCG-GUACCCCCaAGGCGGcguc -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 28543 | 0.69 | 0.188089 |
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Target: 5'- aCUGGGC---GGGGGccgUCCGCCGCGc -3' miRNA: 3'- gGGCUCGguaCCCCCa--AGGCGGCGUc -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 16172 | 0.69 | 0.183187 |
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Target: 5'- gCCaGGUgGUGGGGG--CCGCCGCGa -3' miRNA: 3'- gGGcUCGgUACCCCCaaGGCGGCGUc -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 13813 | 0.74 | 0.087616 |
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Target: 5'- gCCC-AGCUugcgGGGGaGUUCCGCCGcCAGa -3' miRNA: 3'- -GGGcUCGGua--CCCC-CAAGGCGGC-GUC- -5' |
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| 23103 | 5' | -61.6 | NC_005178.1 | + | 11766 | 1.1 | 0.000133 |
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Target: 5'- gCCCGAGCCAUGGGGGUUCCGCCGCAGg -3' miRNA: 3'- -GGGCUCGGUACCCCCAAGGCGGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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