Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23107 | 3' | -53.8 | NC_005178.1 | + | 16187 | 0.66 | 0.641334 |
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Target: 5'- --aACCcugCUGCGAGCGGUgaccgGCCuCGGc -3' miRNA: 3'- ugaUGGa--GAUGUUCGCCAa----CGG-GCUc -5' |
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| 23107 | 3' | -53.8 | NC_005178.1 | + | 5402 | 0.67 | 0.618333 |
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Target: 5'- gGCUcACCUCUGCAuGGuCGGggGCaaGAGc -3' miRNA: 3'- -UGA-UGGAGAUGU-UC-GCCaaCGggCUC- -5' |
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| 23107 | 3' | -53.8 | NC_005178.1 | + | 27037 | 0.7 | 0.43225 |
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Target: 5'- cGCUgaGCaggCUACGGGCGGUUuGCCCGu- -3' miRNA: 3'- -UGA--UGga-GAUGUUCGCCAA-CGGGCuc -5' |
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| 23107 | 3' | -53.8 | NC_005178.1 | + | 35564 | 0.71 | 0.402733 |
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Target: 5'- cGCgACCUCUgcuGCGAGCGGUU-CCCGc- -3' miRNA: 3'- -UGaUGGAGA---UGUUCGCCAAcGGGCuc -5' |
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| 23107 | 3' | -53.8 | NC_005178.1 | + | 2661 | 0.71 | 0.398894 |
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Target: 5'- -gUGCCUCgauCAGGCGGcgcacagugaucGCCCGAGg -3' miRNA: 3'- ugAUGGAGau-GUUCGCCaa----------CGGGCUC- -5' |
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| 23107 | 3' | -53.8 | NC_005178.1 | + | 13980 | 1.08 | 0.000961 |
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Target: 5'- gACUACCUCUACAAGCGGUUGCCCGAGc -3' miRNA: 3'- -UGAUGGAGAUGUUCGCCAACGGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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