Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23109 | 3' | -66.9 | NC_005178.1 | + | 34830 | 0.71 | 0.06343 |
Target: 5'- cUCgCCCAGGGCggcguucagcaGCgCGGCGcggguuuccuucggcAGGGCCGa -3' miRNA: 3'- aAGgGGGUCCCG-----------CG-GCCGU---------------UCCCGGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 9417 | 0.66 | 0.154674 |
Target: 5'- --gCCCCuGGGCGCCGcCAucGGuGCCa -3' miRNA: 3'- aagGGGGuCCCGCGGCcGUu-CC-CGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 19656 | 0.66 | 0.139032 |
Target: 5'- -cCUgCCAGGcGCGCUGGCGuaccGGGUCc -3' miRNA: 3'- aaGGgGGUCC-CGCGGCCGUu---CCCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 6476 | 0.66 | 0.135355 |
Target: 5'- gUUCCUCCAGGGCaGCaaaacccagcggCGGCGAcgccuGGCCa -3' miRNA: 3'- -AAGGGGGUCCCG-CG------------GCCGUUc----CCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 34741 | 0.66 | 0.133909 |
Target: 5'- -aCCCCCuggcGGGCaucggcgaccaguuGCUGGCGguugguuucgaccAGGGCCa -3' miRNA: 3'- aaGGGGGu---CCCG--------------CGGCCGU-------------UCCCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 6675 | 0.66 | 0.131767 |
Target: 5'- gUCUUCCAGGGCGCucugguggaCGGCuGGGuaCGa -3' miRNA: 3'- aAGGGGGUCCCGCG---------GCCGuUCCcgGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 21172 | 0.67 | 0.121524 |
Target: 5'- cUgCCCCAGGGCGuaGGCGcc-GCCGc -3' miRNA: 3'- aAgGGGGUCCCGCggCCGUuccCGGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 8998 | 0.67 | 0.115112 |
Target: 5'- -gCCCCCGGuGGCG-CGGCGGacGGCCc -3' miRNA: 3'- aaGGGGGUC-CCGCgGCCGUUc-CCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 5784 | 0.68 | 0.109017 |
Target: 5'- -gCCCCCuguAGGucguCGCCGGCAuuaccucGGGCCa -3' miRNA: 3'- aaGGGGG---UCCc---GCGGCCGUu------CCCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 14449 | 0.68 | 0.092501 |
Target: 5'- --gCgCCGGGGUGCCGGagcuggucAGGGCCa -3' miRNA: 3'- aagGgGGUCCCGCGGCCgu------UCCCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 20655 | 0.69 | 0.080578 |
Target: 5'- -gCCUcggCCAGGGCGCCGaGCAGcucGGCCu -3' miRNA: 3'- aaGGG---GGUCCCGCGGC-CGUUc--CCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 20843 | 0.7 | 0.070132 |
Target: 5'- ----aCCAGGGCGCCGGCucaguuGGGCg- -3' miRNA: 3'- aagggGGUCCCGCGGCCGuu----CCCGgc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 26402 | 0.72 | 0.051544 |
Target: 5'- ---gCCCAGGGCGgCGGCcAGGGCg- -3' miRNA: 3'- aaggGGGUCCCGCgGCCGuUCCCGgc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 9368 | 0.73 | 0.036727 |
Target: 5'- -gUCgCCAGGGCGCCGGCcGAGGcggauacaGCCGa -3' miRNA: 3'- aaGGgGGUCCCGCGGCCG-UUCC--------CGGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 14219 | 1.04 | 9.2e-05 |
Target: 5'- cUUCCCCCAGGGCGCCGGCAAGGGCCGc -3' miRNA: 3'- -AAGGGGGUCCCGCGGCCGUUCCCGGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 27872 | 0.66 | 0.154674 |
Target: 5'- -aCCUCCAGGcCGCCcagGGCAAcuGGGUCa -3' miRNA: 3'- aaGGGGGUCCcGCGG---CCGUU--CCCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 28700 | 0.66 | 0.146273 |
Target: 5'- -cCCCgaCCAGauGGCggccgacauguggGCCGGCAAGGGCgGc -3' miRNA: 3'- aaGGG--GGUC--CCG-------------CGGCCGUUCCCGgC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 29416 | 0.66 | 0.140529 |
Target: 5'- aUCCCgCC-GGGCGCUGGCucgguuucacugucgAaggaGGGGCUGc -3' miRNA: 3'- aAGGG-GGuCCCGCGGCCG---------------U----UCCCGGC- -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 33036 | 0.66 | 0.131767 |
Target: 5'- aUCCugggcggcaCCgAGGGCauuCCGGCAGGuGGCCa -3' miRNA: 3'- aAGG---------GGgUCCCGc--GGCCGUUC-CCGGc -5' |
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23109 | 3' | -66.9 | NC_005178.1 | + | 25730 | 0.67 | 0.118278 |
Target: 5'- -gCCUCCAGGcGCGCCuGaaugcugcCAGGGGUCGa -3' miRNA: 3'- aaGGGGGUCC-CGCGGcC--------GUUCCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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