Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23109 | 3' | -66.9 | NC_005178.1 | + | 29416 | 0.66 | 0.140529 |
|
Target: 5'- aUCCCgCC-GGGCGCUGGCucgguuucacugucgAaggaGGGGCUGc -3' miRNA: 3'- aAGGG-GGuCCCGCGGCCG---------------U----UCCCGGC- -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 33036 | 0.66 | 0.131767 |
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Target: 5'- aUCCugggcggcaCCgAGGGCauuCCGGCAGGuGGCCa -3' miRNA: 3'- aAGG---------GGgUCCCGc--GGCCGUUC-CCGGc -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 34741 | 0.66 | 0.133909 |
|
Target: 5'- -aCCCCCuggcGGGCaucggcgaccaguuGCUGGCGguugguuucgaccAGGGCCa -3' miRNA: 3'- aaGGGGGu---CCCG--------------CGGCCGU-------------UCCCGGc -5' |
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| 23109 | 3' | -66.9 | NC_005178.1 | + | 34830 | 0.71 | 0.06343 |
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Target: 5'- cUCgCCCAGGGCggcguucagcaGCgCGGCGcggguuuccuucggcAGGGCCGa -3' miRNA: 3'- aAGgGGGUCCCG-----------CG-GCCGU---------------UCCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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