Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23109 | 5' | -53.4 | NC_005178.1 | + | 32897 | 0.67 | 0.690952 |
Target: 5'- uGUCGGCcauGCCAUUGgccagUugGGUGGuuuuCGGg -3' miRNA: 3'- -CAGCCG---UGGUAGCa----AugCCGCUu---GCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 29675 | 0.68 | 0.597889 |
Target: 5'- aUCGGCGCCG-CGcacagucacggACGGgGGACGGa -3' miRNA: 3'- cAGCCGUGGUaGCaa---------UGCCgCUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 29247 | 0.7 | 0.458277 |
Target: 5'- -gCGGCGCUGUCGUacuUGGCGAgaagACGGa -3' miRNA: 3'- caGCCGUGGUAGCAau-GCCGCU----UGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 28163 | 0.66 | 0.7461 |
Target: 5'- gGUCGGCGCC-UCGgcugUAuccgccucggcCGGCGcccuGGCGAc -3' miRNA: 3'- -CAGCCGUGGuAGCa---AU-----------GCCGC----UUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 26000 | 0.67 | 0.657034 |
Target: 5'- cGUCGGCGCgA-CGUUucaGGCGcugGGCGAu -3' miRNA: 3'- -CAGCCGUGgUaGCAAug-CCGC---UUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 24698 | 0.67 | 0.679691 |
Target: 5'- cUCGGCuuCCGcuugcUGUUuccaGCGGCGAGCGGu -3' miRNA: 3'- cAGCCGu-GGUa----GCAA----UGCCGCUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 24113 | 0.66 | 0.71328 |
Target: 5'- -aUGGCAagGUCGUgaccaagGCGGCGGGCu- -3' miRNA: 3'- caGCCGUggUAGCAa------UGCCGCUUGcu -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 23353 | 0.7 | 0.468617 |
Target: 5'- aUCGGCACCGgggCGUcgcaccaggGCGGCagucGGGCGAu -3' miRNA: 3'- cAGCCGUGGUa--GCAa--------UGCCG----CUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 22285 | 0.69 | 0.531846 |
Target: 5'- cGUCGGgGCCAUCGUcguggucUGCGGcCGGGa-- -3' miRNA: 3'- -CAGCCgUGGUAGCA-------AUGCC-GCUUgcu -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 22274 | 0.66 | 0.71328 |
Target: 5'- -cUGGCccGCCAg----GCGGCGGGCGAu -3' miRNA: 3'- caGCCG--UGGUagcaaUGCCGCUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 21819 | 0.66 | 0.724323 |
Target: 5'- cGUCGGCcuucucugcgGCCGaggCGUUgcGCGGCGGcuCGAu -3' miRNA: 3'- -CAGCCG----------UGGUa--GCAA--UGCCGCUu-GCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 20975 | 0.83 | 0.078703 |
Target: 5'- -cCGGCGCCAUCGUcgUGGCGAACGc -3' miRNA: 3'- caGCCGUGGUAGCAauGCCGCUUGCu -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 19985 | 0.67 | 0.63428 |
Target: 5'- uUCGGCACCGgcaGUUGuagccauuCGGCGGcAUGAu -3' miRNA: 3'- cAGCCGUGGUag-CAAU--------GCCGCU-UGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 17869 | 0.66 | 0.750399 |
Target: 5'- -cCGGCGCCAUCuucuggccaagcccuGgcacgcggAUGGCGGugGAg -3' miRNA: 3'- caGCCGUGGUAG---------------Caa------UGCCGCUugCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 16810 | 0.67 | 0.657034 |
Target: 5'- -cCGGCGCUcugcuGUUGggucaggGCGGCGAugGGc -3' miRNA: 3'- caGCCGUGG-----UAGCaa-----UGCCGCUugCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 16350 | 0.75 | 0.246284 |
Target: 5'- uUCGGUGCCGUcCGUgGCGGCGA-CGGu -3' miRNA: 3'- cAGCCGUGGUA-GCAaUGCCGCUuGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 14255 | 1.09 | 0.001042 |
Target: 5'- aGUCGGCACCAUCGUUACGGCGAACGAu -3' miRNA: 3'- -CAGCCGUGGUAGCAAUGCCGCUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 13164 | 0.66 | 0.724323 |
Target: 5'- gGUCGGCugCGgc---GCGGUGAACa- -3' miRNA: 3'- -CAGCCGugGUagcaaUGCCGCUUGcu -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 12563 | 0.73 | 0.336299 |
Target: 5'- aUCgGGCGCCAcag--GCGGCGGACGAa -3' miRNA: 3'- cAG-CCGUGGUagcaaUGCCGCUUGCU- -5' |
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23109 | 5' | -53.4 | NC_005178.1 | + | 8990 | 0.66 | 0.71328 |
Target: 5'- -cCGGCGacgcccCCggUGgcGCGGCGGACGGc -3' miRNA: 3'- caGCCGU------GGuaGCaaUGCCGCUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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