Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23111 | 5' | -57.7 | NC_005178.1 | + | 25784 | 0.72 | 0.168183 |
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Target: 5'- cUGCGGCGgaACCCCCaUGgcuCGGGCCaCGGCc -3' miRNA: 3'- aAUGCCGU--UGGGGG-AC---GCUUGG-GUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 12353 | 0.72 | 0.182793 |
|
Target: 5'- -gGCGGCAaucgcuacagaGCCCCCgGCGAAgCCAu- -3' miRNA: 3'- aaUGCCGU-----------UGGGGGaCGCUUgGGUcg -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 34731 | 0.74 | 0.142071 |
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Target: 5'- -gGCGuGCAgcACCCCCUgGCGGGCaUCGGCg -3' miRNA: 3'- aaUGC-CGU--UGGGGGA-CGCUUG-GGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 10958 | 0.75 | 0.116326 |
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Target: 5'- gUugGGCGGCCCCa-GCGAAUCCcuuGCu -3' miRNA: 3'- aAugCCGUUGGGGgaCGCUUGGGu--CG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 16545 | 0.76 | 0.094986 |
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Target: 5'- -gGCGGUgcuGCCgCCCacgGCGAACCCGGUa -3' miRNA: 3'- aaUGCCGu--UGG-GGGa--CGCUUGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 27966 | 0.76 | 0.097791 |
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Target: 5'- -aGCGGCGACCgCCCaGCGcAGCgCGGCg -3' miRNA: 3'- aaUGCCGUUGG-GGGaCGC-UUGgGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 33782 | 0.78 | 0.064831 |
|
Target: 5'- -aGCGGCAugguGCCgCCCUugGCGAugGCCCGGCg -3' miRNA: 3'- aaUGCCGU----UGG-GGGA--CGCU--UGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 15461 | 1.1 | 0.000262 |
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Target: 5'- uUUACGGCAACCCCCUGCGAACCCAGCa -3' miRNA: 3'- -AAUGCCGUUGGGGGACGCUUGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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