Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23111 | 5' | -57.7 | NC_005178.1 | + | 10783 | 0.68 | 0.335206 |
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Target: 5'- cUACGccGCcGCCaUCCUGgcCGAGCCCAGCc -3' miRNA: 3'- aAUGC--CGuUGG-GGGAC--GCUUGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 9910 | 0.66 | 0.435501 |
|
Target: 5'- -aGCGGCGGCacguugaCCCUGgaGGGCC-AGCg -3' miRNA: 3'- aaUGCCGUUGg------GGGACg-CUUGGgUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 8990 | 0.69 | 0.295533 |
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Target: 5'- --cCGGCGacGCCCCCgguggcgcgGCGGacggcccccGCCCAGUc -3' miRNA: 3'- aauGCCGU--UGGGGGa--------CGCU---------UGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 8111 | 0.68 | 0.343594 |
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Target: 5'- --uUGGCAGCCCCUccuucgacaGUGAaaccgagccagcGCCCGGCg -3' miRNA: 3'- aauGCCGUUGGGGGa--------CGCU------------UGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 7645 | 0.66 | 0.435501 |
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Target: 5'- --uCGGCGACCUCCagcGCGAgcugGCCCGu- -3' miRNA: 3'- aauGCCGUUGGGGGa--CGCU----UGGGUcg -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 6707 | 0.66 | 0.445451 |
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Target: 5'- ---gGGCGGCCUCaCUGCuGAGCCaggaGGUg -3' miRNA: 3'- aaugCCGUUGGGG-GACG-CUUGGg---UCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 5778 | 0.66 | 0.415995 |
|
Target: 5'- --cUGGCcGCCCCCUGUaGGucguCgCCGGCa -3' miRNA: 3'- aauGCCGuUGGGGGACG-CUu---G-GGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 3022 | 0.68 | 0.310949 |
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Target: 5'- -gACGaGCGcCgCCCCUGCG-ACgCAGCg -3' miRNA: 3'- aaUGC-CGUuG-GGGGACGCuUGgGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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