Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23111 | 5' | -57.7 | NC_005178.1 | + | 22898 | 0.71 | 0.20401 |
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Target: 5'- -gGCGGCGGCgcgCUCCUGguaGAACuCCAGCg -3' miRNA: 3'- aaUGCCGUUG---GGGGACg--CUUG-GGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 14026 | 0.71 | 0.20401 |
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Target: 5'- -aGCGGaguGACgCCCUGaucagGAACCCAGCc -3' miRNA: 3'- aaUGCCg--UUGgGGGACg----CUUGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 14852 | 0.69 | 0.273534 |
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Target: 5'- --uCGGCAGCgaCCCgGCGA-UCCAGCu -3' miRNA: 3'- aauGCCGUUGg-GGGaCGCUuGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 35684 | 0.69 | 0.273534 |
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Target: 5'- -cAgGGcCAACUCCUcaCGAACCCAGCg -3' miRNA: 3'- aaUgCC-GUUGGGGGacGCUUGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 8990 | 0.69 | 0.295533 |
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Target: 5'- --cCGGCGacGCCCCCgguggcgcgGCGGacggcccccGCCCAGUc -3' miRNA: 3'- aauGCCGU--UGGGGGa--------CGCU---------UGGGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 20457 | 0.69 | 0.303166 |
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Target: 5'- -aAUGGCgAACCCCUUGCGc-UCCAGg -3' miRNA: 3'- aaUGCCG-UUGGGGGACGCuuGGGUCg -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 3022 | 0.68 | 0.310949 |
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Target: 5'- -gACGaGCGcCgCCCCUGCG-ACgCAGCg -3' miRNA: 3'- aaUGC-CGUuG-GGGGACGCuUGgGUCG- -5' |
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| 23111 | 5' | -57.7 | NC_005178.1 | + | 33782 | 0.78 | 0.064831 |
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Target: 5'- -aGCGGCAugguGCCgCCCUugGCGAugGCCCGGCg -3' miRNA: 3'- aaUGCCGU----UGG-GGGA--CGCU--UGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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