Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23112 | 3' | -56.5 | NC_005178.1 | + | 16983 | 0.66 | 0.484112 |
Target: 5'- cGAGAGgucagCGCC-GCgACCUGGCCc- -3' miRNA: 3'- uUUCUCaa---GCGGuUGgUGGGCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 9422 | 0.66 | 0.462993 |
Target: 5'- --uGGGcgcCGCCAucggugccACCGCCCaGGCCAAa -3' miRNA: 3'- uuuCUCaa-GCGGU--------UGGUGGG-CCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 29380 | 0.66 | 0.452616 |
Target: 5'- cGAGGAuUUCGCgAACgACCUGGUCGu -3' miRNA: 3'- -UUUCUcAAGCGgUUGgUGGGCCGGUu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 26948 | 0.66 | 0.450556 |
Target: 5'- -cAGGGUggauugcugcggUGCCGGCuCGCUCGGCCGGu -3' miRNA: 3'- uuUCUCAa-----------GCGGUUG-GUGGGCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 2178 | 0.66 | 0.442367 |
Target: 5'- -uGGuGUUCGUCAGCaGCCgGGCCu- -3' miRNA: 3'- uuUCuCAAGCGGUUGgUGGgCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 5435 | 0.67 | 0.432251 |
Target: 5'- -cGGAugUCG-CGACgGCCCGGCCGAc -3' miRNA: 3'- uuUCUcaAGCgGUUGgUGGGCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 13389 | 0.67 | 0.422271 |
Target: 5'- ------aUCGCCAGCCACCaGGUCGg -3' miRNA: 3'- uuucucaAGCGGUUGGUGGgCCGGUu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 8677 | 0.67 | 0.402733 |
Target: 5'- cAAGAcGUUCGCCAuguGCCGCaucgaUGGCCu- -3' miRNA: 3'- uUUCU-CAAGCGGU---UGGUGg----GCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 17637 | 0.67 | 0.393181 |
Target: 5'- --cGAGUccagCGCCGGCaagaCCGGCCAGg -3' miRNA: 3'- uuuCUCAa---GCGGUUGgug-GGCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 5603 | 0.67 | 0.393181 |
Target: 5'- ---cGGaaCGCCGACCGCUCGGCUc- -3' miRNA: 3'- uuucUCaaGCGGUUGGUGGGCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 17103 | 0.68 | 0.365425 |
Target: 5'- cAAGGGGUUCGCCAuuACCuggaaCUGGCaCGAu -3' miRNA: 3'- -UUUCUCAAGCGGU--UGGug---GGCCG-GUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 2618 | 0.68 | 0.356478 |
Target: 5'- -uGGAGaUUUGCCGgaucgAUCACgCCGGCCAGu -3' miRNA: 3'- uuUCUC-AAGCGGU-----UGGUG-GGCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 10072 | 0.68 | 0.356478 |
Target: 5'- -uGGAGcuaCGCCGACCauaugGCCCGGCgCAu -3' miRNA: 3'- uuUCUCaa-GCGGUUGG-----UGGGCCG-GUu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 28571 | 0.69 | 0.330572 |
Target: 5'- -cGGGGgcgUCGCCGGCauuCCCGcGCCAu -3' miRNA: 3'- uuUCUCa--AGCGGUUGgu-GGGC-CGGUu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 22585 | 0.69 | 0.306079 |
Target: 5'- cAGGAGUUCGacaugagCGACCAcacCCUGGCCAu -3' miRNA: 3'- uUUCUCAAGCg------GUUGGU---GGGCCGGUu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 35450 | 0.7 | 0.283001 |
Target: 5'- ----cGggCGCCAAgguaagccguacCCGCCCGGCCAGu -3' miRNA: 3'- uuucuCaaGCGGUU------------GGUGGGCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 21003 | 0.7 | 0.283001 |
Target: 5'- --cGGGUUCGCCGugggcggcagcACCGCCacCGGCCu- -3' miRNA: 3'- uuuCUCAAGCGGU-----------UGGUGG--GCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 16797 | 0.7 | 0.283001 |
Target: 5'- cAGGuGcUgGCCAGCCugCCGGCCc- -3' miRNA: 3'- uUUCuCaAgCGGUUGGugGGCCGGuu -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 23203 | 0.7 | 0.261323 |
Target: 5'- cAGGucgUCGCCGACCACCaGGUCAGg -3' miRNA: 3'- uUUCucaAGCGGUUGGUGGgCCGGUU- -5' |
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23112 | 3' | -56.5 | NC_005178.1 | + | 32578 | 0.73 | 0.167785 |
Target: 5'- ---cAGUUCGUCAucACCGCaCCGGCCGAc -3' miRNA: 3'- uuucUCAAGCGGU--UGGUG-GGCCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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