Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23113 | 5' | -60.4 | NC_005178.1 | + | 33508 | 0.66 | 0.316224 |
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Target: 5'- cUCGCGGGCGAaguagcGGUugccgaugaACUCCAGuUCGc- -3' miRNA: 3'- uAGCGCCCGCU------CCA---------UGAGGUC-GGCaa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 24702 | 0.67 | 0.285982 |
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Target: 5'- aGUCGgGGGCGAggaccuGGUugUCCgcgAGCUGa- -3' miRNA: 3'- -UAGCgCCCGCU------CCAugAGG---UCGGCaa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 21397 | 0.68 | 0.244965 |
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Target: 5'- uUCGuCGGGCGugccgggcGGGUACUUCccgAGCCGg- -3' miRNA: 3'- uAGC-GCCCGC--------UCCAUGAGG---UCGGCaa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 17309 | 0.68 | 0.226353 |
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Target: 5'- gGUCGCGGccgcucggcguGCGAGagGCUCCAGaCCGUc -3' miRNA: 3'- -UAGCGCC-----------CGCUCcaUGAGGUC-GGCAa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 30109 | 0.71 | 0.138019 |
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Target: 5'- --aGCGGGUGAuguaacgGGUGCUgCCGGCCGa- -3' miRNA: 3'- uagCGCCCGCU-------CCAUGA-GGUCGGCaa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 30517 | 0.72 | 0.12721 |
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Target: 5'- -aUGUGGGCGAGGUGCcggacuUCgGGCUGUg -3' miRNA: 3'- uaGCGCCCGCUCCAUG------AGgUCGGCAa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 24145 | 0.77 | 0.051926 |
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Target: 5'- -gUGaCGGGCGAGGUACUCCccGCCGa- -3' miRNA: 3'- uaGC-GCCCGCUCCAUGAGGu-CGGCaa -5' |
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| 23113 | 5' | -60.4 | NC_005178.1 | + | 15971 | 1.02 | 0.000478 |
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Target: 5'- cAUCGCGGGCGAGGUACUCCAGCCGUUc -3' miRNA: 3'- -UAGCGCCCGCUCCAUGAGGUCGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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