Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23114 | 3' | -49.9 | NC_005178.1 | + | 27062 | 0.66 | 0.890483 |
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Target: 5'- cCCGUcacgcaagcCCUGUUCgAAGCGCUcgacgauCUCCAg -3' miRNA: 3'- -GGCAu--------GGACAAG-UUCGUGAu------GAGGUg -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 5801 | 0.67 | 0.829429 |
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Target: 5'- gCCGgcauUACCUcGggcCAGGCGCUggGCUCCAUu -3' miRNA: 3'- -GGC----AUGGA-Caa-GUUCGUGA--UGAGGUG- -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 19029 | 0.69 | 0.72337 |
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Target: 5'- gCGU-CCUGauccagUCGGGCACUACUCa-- -3' miRNA: 3'- gGCAuGGACa-----AGUUCGUGAUGAGgug -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 5769 | 0.69 | 0.711968 |
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Target: 5'- aCCGUgucgcccagcACC-GUUC-AGCGCUACUUCAUc -3' miRNA: 3'- -GGCA----------UGGaCAAGuUCGUGAUGAGGUG- -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 22788 | 0.69 | 0.710822 |
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Target: 5'- gCCG-ACCUGgUCGAGCAgUACggcgaggcguccaUCCGCc -3' miRNA: 3'- -GGCaUGGACaAGUUCGUgAUG-------------AGGUG- -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 31969 | 0.7 | 0.700475 |
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Target: 5'- uCCGUaGCCUGgUCuauGCACUGCgcggCCAg -3' miRNA: 3'- -GGCA-UGGACaAGuu-CGUGAUGa---GGUg -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 9808 | 0.75 | 0.398886 |
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Target: 5'- aCCGUACUggcucgccagGUcCAGGCGCUGCUgCGCg -3' miRNA: 3'- -GGCAUGGa---------CAaGUUCGUGAUGAgGUG- -5' |
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| 23114 | 3' | -49.9 | NC_005178.1 | + | 16073 | 1.13 | 0.001141 |
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Target: 5'- gCCGUACCUGUUCAAGCACUACUCCACg -3' miRNA: 3'- -GGCAUGGACAAGUUCGUGAUGAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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