Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23121 | 5' | -55.8 | NC_005178.1 | + | 28944 | 0.66 | 0.557068 |
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Target: 5'- cGUCCGGCaCGGcCAUCGCC-GUGCGcCa -3' miRNA: 3'- -UAGGCUG-GCU-GUAGUGGaUACGCcGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 12789 | 0.66 | 0.557068 |
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Target: 5'- -cUCGACCGGCAgUC-CCUGgaggucGCGGCc -3' miRNA: 3'- uaGGCUGGCUGU-AGuGGAUa-----CGCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 12209 | 0.66 | 0.523922 |
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Target: 5'- -cCUGGCUGGCAuggUCGCCa--GCGGCUu -3' miRNA: 3'- uaGGCUGGCUGU---AGUGGauaCGCCGA- -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 3645 | 0.67 | 0.480974 |
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Target: 5'- -gCCG-CCGAgCAUCGCCgccaggcGCGGCa -3' miRNA: 3'- uaGGCuGGCU-GUAGUGGaua----CGCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 30181 | 0.68 | 0.410292 |
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Target: 5'- -gCUGGCgGGCGUCAUCU--GCGGCg -3' miRNA: 3'- uaGGCUGgCUGUAGUGGAuaCGCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 31891 | 0.68 | 0.400729 |
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Target: 5'- --gUGACCGACGUCGCCgg-GaUGGCg -3' miRNA: 3'- uagGCUGGCUGUAGUGGauaC-GCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 2443 | 0.69 | 0.355107 |
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Target: 5'- -aCCGGCCGcCAgcggCAgCUcgGCGGCg -3' miRNA: 3'- uaGGCUGGCuGUa---GUgGAuaCGCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 13538 | 0.72 | 0.234654 |
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Target: 5'- uUUCGGCUucCAgcUCGCCUAUGCGGCg -3' miRNA: 3'- uAGGCUGGcuGU--AGUGGAUACGCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 3315 | 0.73 | 0.216299 |
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Target: 5'- -cCCGACCGACGUCuauuCCUGcGaCGGCc -3' miRNA: 3'- uaGGCUGGCUGUAGu---GGAUaC-GCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 19606 | 0.74 | 0.188445 |
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Target: 5'- -gCCGACCGACAUCGUCUGucccUGgGGCg -3' miRNA: 3'- uaGGCUGGCUGUAGUGGAU----ACgCCGa -5' |
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| 23121 | 5' | -55.8 | NC_005178.1 | + | 17976 | 1.06 | 0.000749 |
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Target: 5'- gAUCCGACCGACAUCACCUAUGCGGCUg -3' miRNA: 3'- -UAGGCUGGCUGUAGUGGAUACGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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