Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23124 | 5' | -56.7 | NC_005178.1 | + | 19375 | 1.1 | 0.000375 |
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Target: 5'- cCUGCGCGGGCUGCUCAAGAAAGCCGGc -3' miRNA: 3'- -GACGCGCCCGACGAGUUCUUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 18995 | 0.7 | 0.306166 |
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Target: 5'- -gGCGCGGGUUGUgaUCGAGcgcGCCaGGg -3' miRNA: 3'- gaCGCGCCCGACG--AGUUCuuuCGG-CC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 18404 | 0.68 | 0.40997 |
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Target: 5'- aUGCGCGGcaucGCUGCUgAAcuGuuGGCCGa -3' miRNA: 3'- gACGCGCC----CGACGAgUU--CuuUCGGCc -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 14009 | 0.67 | 0.483955 |
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Target: 5'- cCUGCgggcuGCGGGCcaGCggagugacgcccugaUCAGGAAcccAGCCGGu -3' miRNA: 3'- -GACG-----CGCCCGa-CG---------------AGUUCUU---UCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 13321 | 0.71 | 0.242383 |
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Target: 5'- -gGCGcCGGGCUGCUCGcGcu-GUCGGu -3' miRNA: 3'- gaCGC-GCCCGACGAGUuCuuuCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 10350 | 0.7 | 0.313997 |
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Target: 5'- -cGCGcCGGGCUGCgcuGGccGAGCUGGu -3' miRNA: 3'- gaCGC-GCCCGACGaguUCu-UUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 9655 | 0.66 | 0.53303 |
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Target: 5'- uCUGCGCGGcCggugGCUUcgccGAGCCGGc -3' miRNA: 3'- -GACGCGCCcGa---CGAGuucuUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 9334 | 0.68 | 0.400519 |
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Target: 5'- -aGCgGCGGcGCcGCUCAGGAGcguGCCGc -3' miRNA: 3'- gaCG-CGCC-CGaCGAGUUCUUu--CGGCc -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 8173 | 0.68 | 0.373017 |
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Target: 5'- gCUGCGCucguagGGGCUGCUgAugccccGGu--GCCGGu -3' miRNA: 3'- -GACGCG------CCCGACGAgU------UCuuuCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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