Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23124 | 5' | -56.7 | NC_005178.1 | + | 18995 | 0.7 | 0.306166 |
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Target: 5'- -gGCGCGGGUUGUgaUCGAGcgcGCCaGGg -3' miRNA: 3'- gaCGCGCCCGACG--AGUUCuuuCGG-CC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 26795 | 0.71 | 0.276342 |
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Target: 5'- -cGCGgGGGCUGCgu-AGAGAcGuuGGg -3' miRNA: 3'- gaCGCgCCCGACGaguUCUUU-CggCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 21043 | 0.71 | 0.255531 |
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Target: 5'- -gGCGCGGcgUGCgUCGAGGuuGCCGGa -3' miRNA: 3'- gaCGCGCCcgACG-AGUUCUuuCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 23487 | 0.71 | 0.255531 |
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Target: 5'- gCUGCucgGCcGGCUGCUCAAGGcacugaccGGCUGGg -3' miRNA: 3'- -GACG---CGcCCGACGAGUUCUu-------UCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 13321 | 0.71 | 0.242383 |
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Target: 5'- -gGCGcCGGGCUGCUCGcGcu-GUCGGu -3' miRNA: 3'- gaCGC-GCCCGACGAGUuCuuuCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 28510 | 0.72 | 0.229803 |
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Target: 5'- -aGCGCGGcGCggcauUGCUcCAGGucGAAGCCGGc -3' miRNA: 3'- gaCGCGCC-CG-----ACGA-GUUC--UUUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 20210 | 0.74 | 0.174951 |
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Target: 5'- -gGCGCGGGCUGC-CGAGcuGGgCGa -3' miRNA: 3'- gaCGCGCCCGACGaGUUCuuUCgGCc -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 33196 | 0.75 | 0.143801 |
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Target: 5'- gCUGCGCgaccGGaGUUGCUCGGGGgugaucgugccgAGGCCGGg -3' miRNA: 3'- -GACGCG----CC-CGACGAGUUCU------------UUCGGCC- -5' |
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| 23124 | 5' | -56.7 | NC_005178.1 | + | 19375 | 1.1 | 0.000375 |
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Target: 5'- cCUGCGCGGGCUGCUCAAGAAAGCCGGc -3' miRNA: 3'- -GACGCGCCCGACGAGUUCUUUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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