Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23125 | 3' | -57.9 | NC_005178.1 | + | 36129 | 0.66 | 0.467525 |
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Target: 5'- cUGGcgGGUGCaggugGCGAACGagGCCaCGCCu -3' miRNA: 3'- -ACC--UCACGgaa--CGCUUGC--UGG-GCGGu -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 20214 | 0.66 | 0.446385 |
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Target: 5'- cGGGcUGCCgaGCuGGGCGAccucggcCCCGCCGu -3' miRNA: 3'- aCCUcACGGaaCG-CUUGCU-------GGGCGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 14139 | 0.66 | 0.437489 |
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Target: 5'- aUGGA-UGCCUU-CGAACaGgCCGCCAc -3' miRNA: 3'- -ACCUcACGGAAcGCUUGcUgGGCGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 21206 | 0.67 | 0.418092 |
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Target: 5'- cUGGucUGgCUgGUGAGCGugCCGCCc -3' miRNA: 3'- -ACCucACgGAaCGCUUGCugGGCGGu -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 12092 | 0.67 | 0.389086 |
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Target: 5'- gUGGAGUggucgcguggcggGCCUgGcCGAugGGCgCGCCGc -3' miRNA: 3'- -ACCUCA-------------CGGAaC-GCUugCUGgGCGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 6692 | 0.67 | 0.380916 |
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Target: 5'- gGGAGcGCCUggacCGGGCGGCCUcacugcugaGCCAg -3' miRNA: 3'- aCCUCaCGGAac--GCUUGCUGGG---------CGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 11225 | 0.71 | 0.212187 |
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Target: 5'- -uGAGUGCCguucgcccugGCGAGUGACgCCGCCAu -3' miRNA: 3'- acCUCACGGaa--------CGCUUGCUG-GGCGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 19899 | 0.73 | 0.170604 |
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Target: 5'- aUGGAuGUGCCgggcugGCGGAuCGAUgCCGCCAg -3' miRNA: 3'- -ACCU-CACGGaa----CGCUU-GCUG-GGCGGU- -5' |
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| 23125 | 3' | -57.9 | NC_005178.1 | + | 19520 | 1.08 | 0.00037 |
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Target: 5'- cUGGAGUGCCUUGCGAACGACCCGCCAu -3' miRNA: 3'- -ACCUCACGGAACGCUUGCUGGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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