Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23128 | 3' | -47.5 | NC_005178.1 | + | 30242 | 0.66 | 0.968451 |
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Target: 5'- gCGGACCCccaugcgcuGGAGCAGccgGGCGGc -3' miRNA: 3'- -GUCUGGGauuucuu--UCUCGUC---UUGCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 23339 | 0.67 | 0.941297 |
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Target: 5'- cCGGcGCCCUGGGGGAAGuccauGGcCAGGcGCGGa -3' miRNA: 3'- -GUC-UGGGAUUUCUUUC-----UC-GUCU-UGCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 7390 | 0.67 | 0.929843 |
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Target: 5'- cCAGcUCCUGGAGAAAG-GCuaccuGAugGGc -3' miRNA: 3'- -GUCuGGGAUUUCUUUCuCGu----CUugCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 8901 | 0.68 | 0.923629 |
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Target: 5'- cCAGACCCUGGGcc---AGCAGGAuCGGc -3' miRNA: 3'- -GUCUGGGAUUUcuuucUCGUCUU-GCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 36687 | 0.69 | 0.871171 |
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Target: 5'- uCAGACaCUUAcGGAAAG-GCGGAACa- -3' miRNA: 3'- -GUCUG-GGAUuUCUUUCuCGUCUUGcc -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 1761 | 0.69 | 0.853457 |
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Target: 5'- gCGGACCCgcGAGGAAGcgagccGGCAGGcACGu -3' miRNA: 3'- -GUCUGGGauUUCUUUC------UCGUCU-UGCc -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 11415 | 0.71 | 0.772628 |
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Target: 5'- -cGGCCCUGcgccuGuuuGAGCGGGACGGc -3' miRNA: 3'- guCUGGGAUuu---CuuuCUCGUCUUGCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 26182 | 0.71 | 0.76934 |
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Target: 5'- gCAGACCggcaaucaccAGGAGCGGAGCGGa -3' miRNA: 3'- -GUCUGGgauuucu---UUCUCGUCUUGCC- -5' |
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| 23128 | 3' | -47.5 | NC_005178.1 | + | 19996 | 1.11 | 0.003098 |
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Target: 5'- cCAGACCCUAAAGAAAGAGCAGAACGGc -3' miRNA: 3'- -GUCUGGGAUUUCUUUCUCGUCUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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