Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23128 | 5' | -60.3 | NC_005178.1 | + | 12162 | 0.7 | 0.194434 |
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Target: 5'- cGCCUGCUgGUCGGCUGUcUCCcccaaaccGGACa -3' miRNA: 3'- -CGGGCGA-CGGCCGGCAaAGGa-------CCUGa -5' |
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| 23128 | 5' | -60.3 | NC_005178.1 | + | 9022 | 0.71 | 0.156652 |
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Target: 5'- cGCgCCGUUGCCGGCUucgaCCUGGAg- -3' miRNA: 3'- -CG-GGCGACGGCCGGcaaaGGACCUga -5' |
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| 23128 | 5' | -60.3 | NC_005178.1 | + | 2716 | 0.72 | 0.136538 |
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Target: 5'- cGCCgCGCUGCUGaacGCCGc--CCUGGGCg -3' miRNA: 3'- -CGG-GCGACGGC---CGGCaaaGGACCUGa -5' |
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| 23128 | 5' | -60.3 | NC_005178.1 | + | 16654 | 0.73 | 0.112669 |
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Target: 5'- uCCCGCUGCCaGCCaauggcgaggcggUCCUGGGCg -3' miRNA: 3'- cGGGCGACGGcCGGcaa----------AGGACCUGa -5' |
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| 23128 | 5' | -60.3 | NC_005178.1 | + | 23482 | 0.73 | 0.106215 |
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Target: 5'- gGCCCGCUGCuCGGCCGgcugcUCaaGGcACUg -3' miRNA: 3'- -CGGGCGACG-GCCGGCaa---AGgaCC-UGA- -5' |
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| 23128 | 5' | -60.3 | NC_005178.1 | + | 20031 | 1.09 | 0.000203 |
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Target: 5'- cGCCCGCUGCCGGCCGUUUCCUGGACUu -3' miRNA: 3'- -CGGGCGACGGCCGGCAAAGGACCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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