Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23129 | 5' | -59.2 | NC_005178.1 | + | 1696 | 0.66 | 0.421184 |
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Target: 5'- uUCGGCCCCaUCgUCAgGCUcaugcggaaACGCCUCc -3' miRNA: 3'- -AGUCGGGGcAGaAGU-CGA---------UGCGGGGc -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 4743 | 0.66 | 0.421184 |
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Target: 5'- cCGGCCUgGUCUgCuuCUACGgCCCGu -3' miRNA: 3'- aGUCGGGgCAGAaGucGAUGCgGGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 9254 | 0.66 | 0.393306 |
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Target: 5'- gCGGCCCCGg---CAGCUcccGCaGCgCCGg -3' miRNA: 3'- aGUCGGGGCagaaGUCGA---UG-CGgGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 25370 | 0.67 | 0.366653 |
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Target: 5'- -uGGa-CCGUCUUCAGUUGUGCCgCCGa -3' miRNA: 3'- agUCggGGCAGAAGUCGAUGCGG-GGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 28172 | 0.67 | 0.349589 |
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Target: 5'- cUCGGCUguauCCGcC-UCGGCcgGCGCCCUGg -3' miRNA: 3'- -AGUCGG----GGCaGaAGUCGa-UGCGGGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 29556 | 0.69 | 0.259295 |
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Target: 5'- gUCAGCCCCGg---CAGCcccuggACGgCCUCGg -3' miRNA: 3'- -AGUCGGGGCagaaGUCGa-----UGC-GGGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 20235 | 0.71 | 0.176122 |
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Target: 5'- cUCGGCCCCGcCgucCGGCgcaaccacuaucugACGCCCCc -3' miRNA: 3'- -AGUCGGGGCaGaa-GUCGa-------------UGCGGGGc -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 1361 | 0.72 | 0.164964 |
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Target: 5'- cCAGCUCCGUC--CAGCcACugGCCCCGa -3' miRNA: 3'- aGUCGGGGCAGaaGUCGaUG--CGGGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 10735 | 0.75 | 0.094226 |
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Target: 5'- aCAGCCCCG-CUggccaCAGC-GCGUCCCGg -3' miRNA: 3'- aGUCGGGGCaGAa----GUCGaUGCGGGGC- -5' |
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| 23129 | 5' | -59.2 | NC_005178.1 | + | 20147 | 1.09 | 0.000225 |
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Target: 5'- uUCAGCCCCGUCUUCAGCUACGCCCCGg -3' miRNA: 3'- -AGUCGGGGCAGAAGUCGAUGCGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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