Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23134 | 3' | -64.1 | NC_005178.1 | + | 36166 | 0.66 | 0.193999 |
Target: 5'- -uCGUCUUUGCGGCGggccauguucaaGCCCUCGCUuCg -3' miRNA: 3'- uuGCGGAAGCGCCGC------------CGGGGGUGGuG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 32270 | 0.69 | 0.122648 |
Target: 5'- cGCGCCggCccugaacgGUGGCGGCCUCgGCCAa -3' miRNA: 3'- uUGCGGaaG--------CGCCGCCGGGGgUGGUg -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 31413 | 0.68 | 0.156736 |
Target: 5'- --aGCCUUCGCGGCG-CUaCCGCCu- -3' miRNA: 3'- uugCGGAAGCGCCGCcGGgGGUGGug -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 31139 | 0.68 | 0.156736 |
Target: 5'- -uUGUCaUCGCGGC-GCgCCUCGCCGCg -3' miRNA: 3'- uuGCGGaAGCGCCGcCG-GGGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 28531 | 0.68 | 0.156314 |
Target: 5'- cGGCGCuacaugaCUggGCGGgGGCCgUCCGCCGCg -3' miRNA: 3'- -UUGCG-------GAagCGCCgCCGG-GGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 26162 | 0.7 | 0.092918 |
Target: 5'- uAGCGCCUUuaucaucaucggCGCGGCGGUggCCGCUGCg -3' miRNA: 3'- -UUGCGGAA------------GCGCCGCCGggGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 25781 | 0.68 | 0.144514 |
Target: 5'- --gGCCU--GCGGCGGaaCCCCCAUgGCu -3' miRNA: 3'- uugCGGAagCGCCGCC--GGGGGUGgUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 25429 | 0.68 | 0.13574 |
Target: 5'- uGCGUCggCGCaGGCGGCgCgcccaucggccaggCCCGCCACg -3' miRNA: 3'- uUGCGGaaGCG-CCGCCG-G--------------GGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 22584 | 0.73 | 0.062578 |
Target: 5'- aGGCGCUUgCGgaUGGCGGCCUCgACCACg -3' miRNA: 3'- -UUGCGGAaGC--GCCGCCGGGGgUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 21372 | 1.05 | 0.000139 |
Target: 5'- cAACGCCUUCGCGGCGGCCCCCACCACc -3' miRNA: 3'- -UUGCGGAAGCGCCGCCGGGGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 20994 | 0.67 | 0.161014 |
Target: 5'- gAACGCUaccgggUUCGCcgugGGCGGCagcaCCGCCACc -3' miRNA: 3'- -UUGCGG------AAGCG----CCGCCGgg--GGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 20463 | 0.67 | 0.159719 |
Target: 5'- gAACcCCUUgCGCuccagguacucaauGGCGGCCUCCgGCCGCa -3' miRNA: 3'- -UUGcGGAA-GCG--------------CCGCCGGGGG-UGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 20242 | 0.69 | 0.131 |
Target: 5'- -cCGCCgUC-CGGCGcaaccacuaucugacGCCCCCACCAg -3' miRNA: 3'- uuGCGGaAGcGCCGC---------------CGGGGGUGGUg -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 20136 | 0.66 | 0.204457 |
Target: 5'- --gGCCUcguaUGCGGCGGCauagcgCCCgGCCAUg -3' miRNA: 3'- uugCGGAa---GCGCCGCCG------GGGgUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 16940 | 0.67 | 0.179192 |
Target: 5'- cAGCGCCcUgGCGGauGCCCuCCAUCGu -3' miRNA: 3'- -UUGCGGaAgCGCCgcCGGG-GGUGGUg -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 12083 | 0.69 | 0.118008 |
Target: 5'- cAGCGCCUgguggaguggucgCGUGGCGGgCCUgGCCGa -3' miRNA: 3'- -UUGCGGAa------------GCGCCGCCgGGGgUGGUg -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 11932 | 0.69 | 0.119317 |
Target: 5'- gGugGCUUUCGCcGCaGCCUCgGCCGCu -3' miRNA: 3'- -UugCGGAAGCGcCGcCGGGGgUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 11362 | 0.73 | 0.059113 |
Target: 5'- cAGCGCCacgccCGCaGCGGCCaCCGCCGCg -3' miRNA: 3'- -UUGCGGaa---GCGcCGCCGGgGGUGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 10931 | 0.66 | 0.193489 |
Target: 5'- cGCGCCgagUCGaucCGGCGcggcccgccuccuGCCCCCcgcgucACCACa -3' miRNA: 3'- uUGCGGa--AGC---GCCGC-------------CGGGGG------UGGUG- -5' |
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23134 | 3' | -64.1 | NC_005178.1 | + | 10885 | 0.66 | 0.193999 |
Target: 5'- gGACGCCUUCGCGaaGGUCaaggcguaUCGCCAg -3' miRNA: 3'- -UUGCGGAAGCGCcgCCGGg-------GGUGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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