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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23136 | 3' | -53.4 | NC_005178.1 | + | 22089 | 1.04 | 0.001669 |
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Target: 5'- gUUUCCCAGGUCAACCUGGAUUCGGAUg -3' miRNA: 3'- -AAAGGGUCCAGUUGGACCUAAGCCUA- -5' |
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| 23136 | 3' | -53.4 | NC_005178.1 | + | 5942 | 0.69 | 0.450588 |
|
Target: 5'- --cCCCAGGU-GGCCUGGAcggggCGGAc -3' miRNA: 3'- aaaGGGUCCAgUUGGACCUaa---GCCUa -5' |
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| 23136 | 3' | -53.4 | NC_005178.1 | + | 22690 | 0.67 | 0.57245 |
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Target: 5'- --gCCCAGGcCGAgCUGGAUcgcCGGGUc -3' miRNA: 3'- aaaGGGUCCaGUUgGACCUAa--GCCUA- -5' |
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| 23136 | 3' | -53.4 | NC_005178.1 | + | 17215 | 0.67 | 0.618988 |
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Target: 5'- --gCCCuGGUCGauaACCUGGAacguggCGGAa -3' miRNA: 3'- aaaGGGuCCAGU---UGGACCUaa----GCCUa -5' |
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| 23136 | 3' | -53.4 | NC_005178.1 | + | 14778 | 0.67 | 0.560928 |
|
Target: 5'- ---aCCAGGUCGGCCUGGGUg----- -3' miRNA: 3'- aaagGGUCCAGUUGGACCUAagccua -5' |
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| 23136 | 3' | -53.4 | NC_005178.1 | + | 9686 | 0.67 | 0.618988 |
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Target: 5'- --cCCUGGG-CGGCCUGGAggUCGGc- -3' miRNA: 3'- aaaGGGUCCaGUUGGACCUa-AGCCua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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