Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23137 | 5' | -55.6 | NC_005178.1 | + | 16811 | 0.66 | 0.598507 |
Target: 5'- cGGCGCUCUgcuguugGGUCAgggCGGcgaugggcuuggccuGGCCCAGGu -3' miRNA: 3'- -UUGCGAGG-------CUAGUa--GCU---------------UCGGGUCCc -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 2693 | 0.66 | 0.595128 |
Target: 5'- cGAgGCgUCCGGUCGggCGGaauccAGCCCGGuGGu -3' miRNA: 3'- -UUgCG-AGGCUAGUa-GCU-----UCGGGUC-CC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 17413 | 0.66 | 0.583888 |
Target: 5'- gGGCGCUaugCCGccgCAUaCGAGGCC-AGGGa -3' miRNA: 3'- -UUGCGA---GGCua-GUA-GCUUCGGgUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 2117 | 0.66 | 0.572689 |
Target: 5'- uGGCGC-CCGGUCAgcgucaggcugUCGGuggcggccAGCgCCGGGGc -3' miRNA: 3'- -UUGCGaGGCUAGU-----------AGCU--------UCG-GGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 28633 | 0.66 | 0.55932 |
Target: 5'- cAGCGCacggaagccgggCCGAUCcugcuGGCCCAGGGu -3' miRNA: 3'- -UUGCGa-----------GGCUAGuagcuUCGGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 30532 | 0.66 | 0.550456 |
Target: 5'- cGACGCUCgacgcgGAUCGUCGAGGgCgAcGGGu -3' miRNA: 3'- -UUGCGAGg-----CUAGUAGCUUCgGgU-CCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 20336 | 0.66 | 0.550456 |
Target: 5'- cACGUUCCaGGUUAUCGA---CCAGGGc -3' miRNA: 3'- uUGCGAGG-CUAGUAGCUucgGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 31910 | 0.67 | 0.539437 |
Target: 5'- uGGCGCUgCCGGUCAUCGGccaucgcugaucGGCau-GGGc -3' miRNA: 3'- -UUGCGA-GGCUAGUAGCU------------UCGgguCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 17852 | 0.67 | 0.517634 |
Target: 5'- gAGCGCcugCCGcccUCggCGAGGUCCAGGa -3' miRNA: 3'- -UUGCGa--GGCu--AGuaGCUUCGGGUCCc -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 28674 | 0.67 | 0.506865 |
Target: 5'- gGAUGUaCCGggCGaUGGAGCCCAGGu -3' miRNA: 3'- -UUGCGaGGCuaGUaGCUUCGGGUCCc -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 3818 | 0.68 | 0.464812 |
Target: 5'- -uCGC-CCGGauugcacaaacuUCAUCGAcuuucuuGCCCAGGGc -3' miRNA: 3'- uuGCGaGGCU------------AGUAGCUu------CGGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 32304 | 0.69 | 0.414935 |
Target: 5'- -cUGCUCCaGGUUAaccgCGAcgcgacggacagAGCCCAGGGa -3' miRNA: 3'- uuGCGAGG-CUAGUa---GCU------------UCGGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 33282 | 0.71 | 0.30189 |
Target: 5'- cACGCUCggcgCGAaucUCGUCGAGGUgagCCAGGGc -3' miRNA: 3'- uUGCGAG----GCU---AGUAGCUUCG---GGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 4524 | 0.72 | 0.265198 |
Target: 5'- cAACGCUCCGGccugccCAUCGAGccggacgccGCCCAGuGGu -3' miRNA: 3'- -UUGCGAGGCUa-----GUAGCUU---------CGGGUC-CC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 14205 | 0.72 | 0.258307 |
Target: 5'- cGACGCcccggugCCGAUC-UCGucGCCCAGGu -3' miRNA: 3'- -UUGCGa------GGCUAGuAGCuuCGGGUCCc -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 28131 | 0.72 | 0.251564 |
Target: 5'- gGugGCaCCGAUgG-CGgcGCCCAGGGg -3' miRNA: 3'- -UugCGaGGCUAgUaGCuuCGGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 5306 | 0.74 | 0.191886 |
Target: 5'- cGCGCUacaUCGGUCGgcUCGAccaggcGGCCCAGGGc -3' miRNA: 3'- uUGCGA---GGCUAGU--AGCU------UCGGGUCCC- -5' |
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23137 | 5' | -55.6 | NC_005178.1 | + | 22656 | 1.07 | 0.000742 |
Target: 5'- cAACGCUCCGAUCAUCGAAGCCCAGGGc -3' miRNA: 3'- -UUGCGAGGCUAGUAGCUUCGGGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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