Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23138 | 5' | -51.8 | NC_005178.1 | + | 19932 | 0.66 | 0.789128 |
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Target: 5'- aGuGCCGCcGCAGUgaucGAGCgc--GCACg -3' miRNA: 3'- cCuCGGCGuUGUCA----CUCGauaaCGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 12806 | 0.66 | 0.789128 |
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Target: 5'- aGGGCCGCgugGugGGUGAGCUGc----- -3' miRNA: 3'- cCUCGGCG---UugUCACUCGAUaacgug -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 25199 | 0.66 | 0.789128 |
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Target: 5'- uGGGCgGCAcguugacggGCGGUuucGAGCUGcUGCAUg -3' miRNA: 3'- cCUCGgCGU---------UGUCA---CUCGAUaACGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 23024 | 0.66 | 0.778554 |
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Target: 5'- --cGCCGCuuccACAGUGGGUUuuucagcagGCGCg -3' miRNA: 3'- ccuCGGCGu---UGUCACUCGAuaa------CGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 7892 | 0.68 | 0.630398 |
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Target: 5'- cGGcGCCGauGCGGUccugGAGCUAcUGCGCa -3' miRNA: 3'- -CCuCGGCguUGUCA----CUCGAUaACGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 28332 | 0.68 | 0.630398 |
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Target: 5'- cGGAGUCguGCAGCGGcGGGCc-UUGCAUg -3' miRNA: 3'- -CCUCGG--CGUUGUCaCUCGauAACGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 28313 | 0.7 | 0.514808 |
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Target: 5'- cGGGGCCGCuGgAGUG-GCUGgugGCGg -3' miRNA: 3'- -CCUCGGCGuUgUCACuCGAUaa-CGUg -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 32211 | 0.71 | 0.479492 |
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Target: 5'- cGGGGCCGCAcCGGUGuacAGCUccagguucagGCGCu -3' miRNA: 3'- -CCUCGGCGUuGUCAC---UCGAuaa-------CGUG- -5' |
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| 23138 | 5' | -51.8 | NC_005178.1 | + | 22757 | 1.12 | 0.000753 |
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Target: 5'- aGGAGCCGCAACAGUGAGCUAUUGCACc -3' miRNA: 3'- -CCUCGGCGUUGUCACUCGAUAACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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