Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23139 | 5' | -45.8 | NC_005178.1 | + | 1625 | 0.66 | 0.98307 |
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Target: 5'- ---cCCAGCGCaacaugCGGAUCAGUUCg -3' miRNA: 3'- uaacGGUUGUGgcaa--GUUUAGUCAAG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 2525 | 0.66 | 0.980634 |
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Target: 5'- -cUGCCGACGCgGUc--GGUCAGUg- -3' miRNA: 3'- uaACGGUUGUGgCAaguUUAGUCAag -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 31913 | 0.66 | 0.974989 |
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Target: 5'- cUUGCCAuCGgCGUUCAGGuUCGGgccUCg -3' miRNA: 3'- uAACGGUuGUgGCAAGUUU-AGUCa--AG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 5280 | 0.67 | 0.964405 |
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Target: 5'- --gGCCGcCACCGUUCAGGgcCGGcgCg -3' miRNA: 3'- uaaCGGUuGUGGCAAGUUUa-GUCaaG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 27890 | 0.67 | 0.957632 |
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Target: 5'- --gGCCGGCucggcgaagccaccgGCCGcgCAGAUCAGaUCg -3' miRNA: 3'- uaaCGGUUG---------------UGGCaaGUUUAGUCaAG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 18577 | 0.68 | 0.934638 |
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Target: 5'- --cGCCGACcgugACCaGgcgCAGAUCGGUUCc -3' miRNA: 3'- uaaCGGUUG----UGG-Caa-GUUUAGUCAAG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 5776 | 0.7 | 0.892557 |
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Target: 5'- --cGcCCAGCACCGUUCAGcgCuacUUCa -3' miRNA: 3'- uaaC-GGUUGUGGCAAGUUuaGuc-AAG- -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 19921 | 0.7 | 0.884358 |
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Target: 5'- cUUGCCGGCGCUGgacUCGAccGUCAGa-- -3' miRNA: 3'- uAACGGUUGUGGCa--AGUU--UAGUCaag -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 25842 | 0.72 | 0.77955 |
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Target: 5'- --cGCCAugGCCGUggccggcauuucggCAGGUCAGUa- -3' miRNA: 3'- uaaCGGUugUGGCAa-------------GUUUAGUCAag -5' |
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| 23139 | 5' | -45.8 | NC_005178.1 | + | 23128 | 1.1 | 0.004509 |
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Target: 5'- cAUUGCCAACACCGUUCAAAUCAGUUCg -3' miRNA: 3'- -UAACGGUUGUGGCAAGUUUAGUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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