Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23140 | 3' | -54.2 | NC_005178.1 | + | 27902 | 0.66 | 0.714852 |
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Target: 5'- aCGGGG-CGACGUCGG-CGUucGCUGAc- -3' miRNA: 3'- -GCUCUaGCUGCAGUCaGCG--CGGCUuc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 20293 | 0.66 | 0.692845 |
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Target: 5'- -aGGAUCGGCcgcaGGUUGCGCUGGAa -3' miRNA: 3'- gcUCUAGCUGcag-UCAGCGCGGCUUc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 11729 | 0.66 | 0.670595 |
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Target: 5'- -----aUGGCGUCAGaCGUGCCGAGc -3' miRNA: 3'- gcucuaGCUGCAGUCaGCGCGGCUUc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 31765 | 0.67 | 0.648194 |
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Target: 5'- gGAGGUCGAUGa-AGUaGCGCUGAAc -3' miRNA: 3'- gCUCUAGCUGCagUCAgCGCGGCUUc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 1532 | 0.68 | 0.558793 |
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Target: 5'- aCGAGGUCGcCGcccgCAuGUCGCGCCu--- -3' miRNA: 3'- -GCUCUAGCuGCa---GU-CAGCGCGGcuuc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 33578 | 0.71 | 0.413504 |
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Target: 5'- uCGAGGcCGACGUgcUGGUCGCGaCCGgcGa -3' miRNA: 3'- -GCUCUaGCUGCA--GUCAGCGC-GGCuuC- -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 9699 | 0.71 | 0.413504 |
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Target: 5'- gGAGGUCGGCG-UAGUUGCGuuGcAGc -3' miRNA: 3'- gCUCUAGCUGCaGUCAGCGCggCuUC- -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 32249 | 0.71 | 0.385458 |
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Target: 5'- gGAGGUCGGCcaGUucCAGcUCGCGCCGGc- -3' miRNA: 3'- gCUCUAGCUG--CA--GUC-AGCGCGGCUuc -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 2294 | 0.72 | 0.376392 |
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Target: 5'- aCGGuAUCGGCG-CAGcCGCGCgCGAGGg -3' miRNA: 3'- -GCUcUAGCUGCaGUCaGCGCG-GCUUC- -5' |
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| 23140 | 3' | -54.2 | NC_005178.1 | + | 23725 | 1.09 | 0.001019 |
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Target: 5'- aCGAGAUCGACGUCAGUCGCGCCGAAGc -3' miRNA: 3'- -GCUCUAGCUGCAGUCAGCGCGGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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