Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23141 | 5' | -53.5 | NC_005178.1 | + | 17140 | 0.66 | 0.682128 |
Target: 5'- cGCGCAGCUuggCCagGUUGGUcgccugggcguccAGGGCCg -3' miRNA: 3'- aUGCGUCGAa--GGgaUAGCCG-------------UUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 37083 | 0.66 | 0.671797 |
Target: 5'- -uCGCuGGCgUCCCgGUCGGC--GACCu -3' miRNA: 3'- auGCG-UCGaAGGGaUAGCCGuuCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 6741 | 0.66 | 0.660278 |
Target: 5'- aUGCGCcgAGCacugaUCCCcAUCGGCAucuuccuGGCCc -3' miRNA: 3'- -AUGCG--UCGa----AGGGaUAGCCGUu------CUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 16377 | 0.66 | 0.660278 |
Target: 5'- gGCgGCGGCgccuacgCCCUGg-GGCAGGuCCa -3' miRNA: 3'- aUG-CGUCGaa-----GGGAUagCCGUUCuGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 37509 | 0.66 | 0.659125 |
Target: 5'- --aGCAGCUcggccgaUUCCUgcgggagggcAUCGGCAAgcuGACCg -3' miRNA: 3'- augCGUCGA-------AGGGA----------UAGCCGUU---CUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 9298 | 0.66 | 0.648731 |
Target: 5'- aUugGCGGCggCCagcgaggCGGCAGcGGCCu -3' miRNA: 3'- -AugCGUCGaaGGgaua---GCCGUU-CUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 35154 | 0.67 | 0.637167 |
Target: 5'- aACGCcagccGGCUggaggacgUCaCUGUCGGCcAGGCCg -3' miRNA: 3'- aUGCG-----UCGA--------AGgGAUAGCCGuUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 32082 | 0.67 | 0.623285 |
Target: 5'- gUGCGCaauaucGGCUaugCCCguuucgcgaaugGUCGGCcGGGCCg -3' miRNA: 3'- -AUGCG------UCGAa--GGGa-----------UAGCCGuUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 17853 | 0.67 | 0.614035 |
Target: 5'- aGCGCcuGCcgCCC--UCGGCGAGGuCCa -3' miRNA: 3'- aUGCGu-CGaaGGGauAGCCGUUCU-GG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 421 | 0.68 | 0.568063 |
Target: 5'- gGCGUAGC-UCCUggaccUCGGCGAGGa- -3' miRNA: 3'- aUGCGUCGaAGGGau---AGCCGUUCUgg -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 33560 | 0.68 | 0.554423 |
Target: 5'- gUGCGCAGCa--CCUGgggucggaacagCGGCcGGGCCu -3' miRNA: 3'- -AUGCGUCGaagGGAUa-----------GCCGuUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 4820 | 0.68 | 0.523003 |
Target: 5'- aUGCgGCAGCUaUCCacgGUCGGCcAGuCCa -3' miRNA: 3'- -AUG-CGUCGA-AGGga-UAGCCGuUCuGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 27732 | 0.69 | 0.511945 |
Target: 5'- cGCGCAGCagCgCCUGgaccUGGCGAG-CCa -3' miRNA: 3'- aUGCGUCGaaG-GGAUa---GCCGUUCuGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 18195 | 0.69 | 0.468757 |
Target: 5'- cUGCGCGGUggCgUCUAUgGGCcGGGCCa -3' miRNA: 3'- -AUGCGUCGaaG-GGAUAgCCGuUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 12768 | 0.7 | 0.458251 |
Target: 5'- cACGCGGCa--CCUGgaggaacgccUCGGCAAuGGCCu -3' miRNA: 3'- aUGCGUCGaagGGAU----------AGCCGUU-CUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 18616 | 0.7 | 0.444783 |
Target: 5'- cUACGCAGCUaUCCagcaGUUgggcggucaggcugGGCGAGGCCg -3' miRNA: 3'- -AUGCGUCGA-AGGga--UAG--------------CCGUUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 21411 | 0.7 | 0.437625 |
Target: 5'- cGgGCGGguaCUUCCCgagccggAUCGGC-AGACCg -3' miRNA: 3'- aUgCGUC---GAAGGGa------UAGCCGuUCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 13321 | 0.7 | 0.417541 |
Target: 5'- gGCGCcgGGCUgcucgCgCUGUCGGUGAuGACCa -3' miRNA: 3'- aUGCG--UCGAa----GgGAUAGCCGUU-CUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 14508 | 0.71 | 0.369884 |
Target: 5'- --aGCAGCUUCCgCUugcgcUCGGCAuccaGGAUCg -3' miRNA: 3'- augCGUCGAAGG-GAu----AGCCGU----UCUGG- -5' |
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23141 | 5' | -53.5 | NC_005178.1 | + | 8590 | 0.72 | 0.351891 |
Target: 5'- cGCGCAGggUCaCCUGgcgcaCGGCGAuGGCCg -3' miRNA: 3'- aUGCGUCgaAG-GGAUa----GCCGUU-CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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