Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23142 | 3' | -65.7 | NC_005178.1 | + | 31243 | 0.66 | 0.170528 |
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Target: 5'- gACAGCaucguCGAGC-GCCUGaGCGCCUCg- -3' miRNA: 3'- -UGUCGc----GCUCGuCGGGC-CGCGGGGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 31305 | 0.71 | 0.073053 |
|
Target: 5'- cCAGCGcCGGGCuaugcGCCUGGCGCCgCg- -3' miRNA: 3'- uGUCGC-GCUCGu----CGGGCCGCGGgGag -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 33016 | 0.66 | 0.179769 |
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Target: 5'- gGCGGCGUccggcucgauGGGCAGgCCGGagcguUGCCgCUCg -3' miRNA: 3'- -UGUCGCG----------CUCGUCgGGCC-----GCGGgGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 33857 | 0.66 | 0.157462 |
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Target: 5'- uCGGCGcCGAuGUAGCCgGGCaGUUCCUUg -3' miRNA: 3'- uGUCGC-GCU-CGUCGGgCCG-CGGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 34425 | 0.69 | 0.099204 |
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Target: 5'- gGCAGCGCGGGC-GCU--GCGCUUCUCc -3' miRNA: 3'- -UGUCGCGCUCGuCGGgcCGCGGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 34839 | 0.7 | 0.08637 |
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Target: 5'- gGCGGCGUucAGCAGCgCGGCGCggguuuCCUUCg -3' miRNA: 3'- -UGUCGCGc-UCGUCGgGCCGCG------GGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 35643 | 0.66 | 0.153312 |
|
Target: 5'- uCAaCGUGAGCcucGGCCUGGCGauaCUCCUCa -3' miRNA: 3'- uGUcGCGCUCG---UCGGGCCGC---GGGGAG- -5' |
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| 23142 | 3' | -65.7 | NC_005178.1 | + | 37492 | 0.66 | 0.161714 |
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Target: 5'- cCAGCGCGAaugcaucgaGCAGCUCGGcCGaUUCCUg -3' miRNA: 3'- uGUCGCGCU---------CGUCGGGCC-GC-GGGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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