Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23143 | 3' | -56.9 | NC_005178.1 | + | 24530 | 1.1 | 0.00039 |
Target: 5'- cCGUCGCCGGUAUGCAGAUCAGCGGCGg -3' miRNA: 3'- -GCAGCGGCCAUACGUCUAGUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 25406 | 0.72 | 0.23672 |
Target: 5'- aCGUCG-CGGUAggcGCGGGUCugugcgucggcgcaGGCGGCGc -3' miRNA: 3'- -GCAGCgGCCAUa--CGUCUAG--------------UCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 25850 | 0.71 | 0.289066 |
Target: 5'- cCGUgGCCGGcauuUcgGCAGGUCAguaccgccaggccauGCGGCa -3' miRNA: 3'- -GCAgCGGCC----AuaCGUCUAGU---------------CGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 30615 | 0.71 | 0.286136 |
Target: 5'- -cUCGCCGGcuuucgucuggcUGcUGCGGGccaccUCAGCGGCGa -3' miRNA: 3'- gcAGCGGCC------------AU-ACGUCU-----AGUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 10277 | 0.7 | 0.301022 |
Target: 5'- --aCGCUGGUGguagucgaGCAGcUCGGCGGCa -3' miRNA: 3'- gcaGCGGCCAUa-------CGUCuAGUCGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 31898 | 0.7 | 0.324461 |
Target: 5'- aCGUCGCCGGgauggcgcUGCcGGUCAuCGGCc -3' miRNA: 3'- -GCAGCGGCCau------ACGuCUAGUcGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 6916 | 0.69 | 0.340827 |
Target: 5'- aGgCGCUGGUGcUGCcGAaaUCGGUGGCGg -3' miRNA: 3'- gCaGCGGCCAU-ACGuCU--AGUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 5503 | 0.69 | 0.366479 |
Target: 5'- gGUCGCCaGU-UGCgAGA-CGGUGGCGa -3' miRNA: 3'- gCAGCGGcCAuACG-UCUaGUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 2446 | 0.68 | 0.402694 |
Target: 5'- gGcCGCCaGcg-GCAGcUCGGCGGCGa -3' miRNA: 3'- gCaGCGGcCauaCGUCuAGUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 32925 | 0.67 | 0.461062 |
Target: 5'- gGUCGCCGGcAUGCcGAUggcugguuuacCGGgGGCu -3' miRNA: 3'- gCAGCGGCCaUACGuCUA-----------GUCgCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 25895 | 0.67 | 0.441098 |
Target: 5'- aGUUGCCGGc--GCAGAUCAccgacGUGGUc -3' miRNA: 3'- gCAGCGGCCauaCGUCUAGU-----CGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 33678 | 0.67 | 0.460053 |
Target: 5'- ---gGCCGGUGUuCGGGUCAacgaugcGCGGCa -3' miRNA: 3'- gcagCGGCCAUAcGUCUAGU-------CGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 2852 | 0.67 | 0.461062 |
Target: 5'- cCG-CGCCGGgucgaacggAUGCGGAaCGGCuGGCc -3' miRNA: 3'- -GCaGCGGCCa--------UACGUCUaGUCG-CCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 5372 | 0.67 | 0.471222 |
Target: 5'- gCGUCcaGCuCGGUGcUGguGAggaugCGGCGGUGa -3' miRNA: 3'- -GCAG--CG-GCCAU-ACguCUa----GUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 29539 | 0.67 | 0.471222 |
Target: 5'- uCGaUGCCGGUGUcaGGGUCAGCcccGGCa -3' miRNA: 3'- -GCaGCGGCCAUAcgUCUAGUCG---CCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 9139 | 0.67 | 0.481495 |
Target: 5'- ---gGCCGGUAUGCugucgcuGGUCGGUGuGCc -3' miRNA: 3'- gcagCGGCCAUACGu------CUAGUCGC-CGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 34524 | 0.66 | 0.534356 |
Target: 5'- gGUCGCUGcGUc-GCAGG--GGCGGCGc -3' miRNA: 3'- gCAGCGGC-CAuaCGUCUagUCGCCGC- -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 12166 | 0.66 | 0.512935 |
Target: 5'- aCGUC-CUGGa--GCAG-UCGGCGGCa -3' miRNA: 3'- -GCAGcGGCCauaCGUCuAGUCGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 33390 | 0.66 | 0.48771 |
Target: 5'- uGUUGCCGuucagcucugcgGUGCAGAggaAGCGGCc -3' miRNA: 3'- gCAGCGGCca----------UACGUCUag-UCGCCGc -5' |
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23143 | 3' | -56.9 | NC_005178.1 | + | 29956 | 0.66 | 0.502357 |
Target: 5'- -cUCGCgGGUcUGCGGGacguagaCGGCGGCa -3' miRNA: 3'- gcAGCGgCCAuACGUCUa------GUCGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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