miRNA: 3'- -GCAgCGGCC----AuaCGUCUAGU---------------CGCCGc -5'
Results 21 - 21 of 21 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23143 | 3' | -56.9 | NC_005178.1 | + | 25850 | 0.71 | 0.289066 |
|
Target: 5'- cCGUgGCCGGcauuUcgGCAGGUCAguaccgccaggccauGCGGCa -3' miRNA: 3'- -GCAgCGGCC----AuaCGUCUAGU---------------CGCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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