Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23145 | 5' | -51.7 | NC_005178.1 | + | 5876 | 0.66 | 0.755642 |
Target: 5'- aGCCGGGU--CAGCguagGCUGUUCg- -3' miRNA: 3'- -UGGCUCGcuGUCGauuaCGACAAGaa -5' |
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23145 | 5' | -51.7 | NC_005178.1 | + | 25102 | 1.05 | 0.002155 |
Target: 5'- cACCGAGCGACAGCUAAUGCUGUUCUUc -3' miRNA: 3'- -UGGCUCGCUGUCGAUUACGACAAGAA- -5' |
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23145 | 5' | -51.7 | NC_005178.1 | + | 31370 | 0.7 | 0.522578 |
Target: 5'- -gCGAGCGGCAGCUacuggacauGAcGCUGUUUg- -3' miRNA: 3'- ugGCUCGCUGUCGA---------UUaCGACAAGaa -5' |
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23145 | 5' | -51.7 | NC_005178.1 | + | 36536 | 0.68 | 0.644403 |
Target: 5'- gGCCGAGCGGUAGCcuugcccgcuacggGGUGgaGUUCUa -3' miRNA: 3'- -UGGCUCGCUGUCGa-------------UUACgaCAAGAa -5' |
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23145 | 5' | -51.7 | NC_005178.1 | + | 24933 | 0.66 | 0.733161 |
Target: 5'- uCCaAGCGACGGUgaguccUGAUGCUGgUCUg -3' miRNA: 3'- uGGcUCGCUGUCG------AUUACGACaAGAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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