Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23146 | 3' | -54.3 | NC_005178.1 | + | 9634 | 0.66 | 0.639826 |
Target: 5'- cGGCUGAUGcacucgaucugaucuGCG--CGGCCGgUGGCUUCg -3' miRNA: 3'- -UCGACUAC---------------CGCcaGUUGGU-ACCGAAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 34764 | 0.66 | 0.635196 |
Target: 5'- cAGUUGcUGGCGGuugguuUCGACCAgGGCc-- -3' miRNA: 3'- -UCGACuACCGCC------AGUUGGUaCCGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 10278 | 0.66 | 0.623619 |
Target: 5'- cGCUGGUGGUaGUCGAgCAgcucGGCggCa -3' miRNA: 3'- uCGACUACCGcCAGUUgGUa---CCGaaG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 15291 | 0.66 | 0.623619 |
Target: 5'- uGGCgGGccagccUGGCGGUCGGCU-UGGCg-- -3' miRNA: 3'- -UCGaCU------ACCGCCAGUUGGuACCGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 35957 | 0.66 | 0.61205 |
Target: 5'- cAGCUcg-GGCGGgacUCGACCA-GGCgUCg -3' miRNA: 3'- -UCGAcuaCCGCC---AGUUGGUaCCGaAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 24955 | 0.66 | 0.6005 |
Target: 5'- uGCUGGucUGGUGGgcagcCAACCAgGGUUUUu -3' miRNA: 3'- uCGACU--ACCGCCa----GUUGGUaCCGAAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 29479 | 0.66 | 0.587831 |
Target: 5'- aAGC-GAUGGU-GUCGGCCAUGucacugcaccuccGCUUCg -3' miRNA: 3'- -UCGaCUACCGcCAGUUGGUAC-------------CGAAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 10099 | 0.67 | 0.577501 |
Target: 5'- aGGCUGAUGcGCuGGgCcGCCGUGGUg-- -3' miRNA: 3'- -UCGACUAC-CG-CCaGuUGGUACCGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 15092 | 0.67 | 0.543399 |
Target: 5'- gAGCUGGUGaaGCGcUCGGCCAaGGCg-- -3' miRNA: 3'- -UCGACUAC--CGCcAGUUGGUaCCGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 4267 | 0.67 | 0.543399 |
Target: 5'- ------aGGCGGUCGGCUugGGCUUCu -3' miRNA: 3'- ucgacuaCCGCCAGUUGGuaCCGAAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 2135 | 0.67 | 0.532173 |
Target: 5'- aGGCUGucgGUGGCGGcCAgcGCCggGGCc-- -3' miRNA: 3'- -UCGAC---UACCGCCaGU--UGGuaCCGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 1676 | 0.68 | 0.506687 |
Target: 5'- uGCUGAccaacUGGCuGGUCGAUgugcguggcggccgCGUGGCUUa -3' miRNA: 3'- uCGACU-----ACCG-CCAGUUG--------------GUACCGAAg -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 17180 | 0.68 | 0.466842 |
Target: 5'- cGGCUucagGGCGGUCAggGCCGcaaUGGCcUCu -3' miRNA: 3'- -UCGAcua-CCGCCAGU--UGGU---ACCGaAG- -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 36070 | 0.7 | 0.36816 |
Target: 5'- cGCUGGUGGgcaaCGGgugCGACCAcGGCUa- -3' miRNA: 3'- uCGACUACC----GCCa--GUUGGUaCCGAag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 28391 | 0.7 | 0.359109 |
Target: 5'- aGGgUGAUGGUGuauaCGGCCAUGGCUa- -3' miRNA: 3'- -UCgACUACCGCca--GUUGGUACCGAag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 28327 | 0.71 | 0.350214 |
Target: 5'- uGGCUGGUGGCGGga----GUGGCUg- -3' miRNA: 3'- -UCGACUACCGCCaguuggUACCGAag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 6547 | 0.71 | 0.316219 |
Target: 5'- cGCUcGAUGGCGGUCAG-CGUGcGCg-- -3' miRNA: 3'- uCGA-CUACCGCCAGUUgGUAC-CGaag -5' |
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23146 | 3' | -54.3 | NC_005178.1 | + | 25169 | 1.11 | 0.000466 |
Target: 5'- gAGCUGAUGGCGGUCAACCAUGGCUUCg -3' miRNA: 3'- -UCGACUACCGCCAGUUGGUACCGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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